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120,922 results • Page
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Votes
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0
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2
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26
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C.reinhardtii single-cell Seurat object - gene names instead of loci
loci
Chlamydomonas
single-cell
10 minutes ago by
Ana
• 0
0
votes
1
reply
22
views
Snakemake Global Environment Container
Snakemake
Conda
Singularity
updated 20 minutes ago by
Ram
45k • written 1 hour ago by
meetmet
• 0
0
votes
0
replies
41
views
GTF repeatmasker annotation file
repeatmasker
GTF
2 hours ago by
frarodmar17
• 0
0
votes
2
replies
1.3k
views
Searching for online tools to prepare mitochondrial codon usage
genome
updated 3 hours ago by
aboozar.soorni
▴ 30 • written 4.6 years ago by
puipui
• 0
1
vote
1
reply
64
views
Getting sequences fragments using pysam
sequencing
pysam
reads
wgs
updated 4 hours ago by
i.sudbery
21k • written 4 hours ago by
Juls
• 0
6
votes
9
replies
2.9k
views
Does EnhancedVolcano() supports crosstalk()?
EnhancedVolcano
crosstalk
R
ggplot
updated 3 hours ago by
GenoMax
150k • written 5.2 years ago by
IrK
▴ 100
0
votes
1
reply
63
views
How to select GEO datasets for differential expression analysis using GEO2R
GEO
DEGs
GEO2R
updated 3 hours ago by
ATpoint
87k • written 5 hours ago by
dreamer247
• 0
0
votes
0
replies
74
views
Blog:
Unlocking the Cellular Universe: The Importance of Single-Cell Sequencing in Animal and Plant Research
Single-cell
6 hours ago by
Novogene
▴ 500
836
votes
169
replies
172k
views
110 follow
News:
The Biostar Handbook. A bioinformatics e-book for beginners.
training
handbook
updated 6 months ago by
Biostar
3.4k • written 8.3 years ago by
Istvan Albert
102k
0
votes
4
replies
640
views
PopGenome lost individual information
PopGenome
updated 1 hour ago by
Arup Ghosh
3.3k • written 12 months ago by
Yixuan
• 0
1
vote
1
reply
108
views
Question About Handling Technical Replicates in ATAC-seq
technical-replicate
atacseq
updated 11 hours ago by
rfran010
★ 1.4k • written 17 hours ago by
LuciaNhu
▴ 10
12
votes
16
replies
18k
views
8 follow
How to convert vcf to 23andme format
genome
sequencing
SNP
updated 15 hours ago by
ngelbal
• 0 • written 7.5 years ago by
alec_djinn
▴ 390
0
votes
4
replies
143
views
Poor alignment rates using Bowtie
aligment
bowtie2
bowtie
miRNAs
updated 40 minutes ago by
GenoMax
150k • written 15 hours ago by
omicon
▴ 40
1
vote
5
replies
136
views
Polishing raw nanopore reads with Pacbio
polishing
assembly
updated 50 minutes ago by
GenoMax
150k • written 17 hours ago by
samuel.a.odonnell
▴ 590
0
votes
4
replies
392
views
bbduk.sh trimming to BAM output file
bbduk.sh
updated 19 hours ago by
Brian Bushnell
20k • written 6 days ago by
bge
• 0
0
votes
3
replies
190
views
Identifying domain of long-read assembled contig
longread
taxonomy
identification
contig
updated 19 hours ago by
Mensur Dlakic
★ 29k • written 1 day ago by
Gio
• 0
0
votes
1
reply
109
views
Methods for directional pathway analysis of DEGs?
Pathway
ORA
GSEA
analysis
Limma
updated 7 hours ago by
Basti
★ 2.0k • written 21 hours ago by
Steven
• 0
0
votes
4
replies
149
views
Discrepancy between positions on IGV and NCBI's PrimerBLAST
igv
primer
blast
1 hour ago by
Rozita
▴ 40
0
votes
0
replies
80
views
Why does Radian run so much slower than vanilla R?
R
Python
Radian
VS-Code
1 day ago by
RR
• 0
4
votes
10
replies
302
views
6 follow
About the Tool FASTP
Quality-control
reads
fastp
updated 1 hour ago by
shelkmike
★ 1.5k • written 1 day ago by
AIMAR
• 0
0
votes
0
replies
64
views
Significant SNPs with the same effect in FarmCPU
effect-size
gwas
FarmCPU
likage-disequilibrium
1 day ago by
AndrMod
• 0
6
votes
11
replies
718
views
Larger genome size than expected-please help
genome
nanopore
size
minimap2
1 day ago by
alexandrakortsi
• 0
0
votes
0
replies
84
views
Job:
Postdoc ML/AI in Genomics & Bioinformatics at Duke University
Postodoc
Genomics
AI
1 day ago by
alwayshope
▴ 40
0
votes
1
reply
126
views
analyze gene expression of 3 genes in RNA-Seq data
R
RNA-Seq
updated 23 hours ago by
ATpoint
87k • written 1 day ago by
pinheirofabiano
▴ 100
1
vote
1
reply
357
views
methRead takes too long
MethylKit
updated 1 day ago by
Alexander Blume
▴ 10 • written 7 months ago by
CTLong
▴ 140
0
votes
0
replies
79
views
rrvgo::getGoSize error
ReViGO
GO-terms
rrvgo
updated 15 hours ago by
Ram
45k • written 1 day ago by
Ana
• 0
0
votes
0
replies
78
views
Genotype Imputation for VCF File via API Using R ?
Genotype-Imputation
VCF
API
1 day ago by
Ahmed Jaber
• 0
0
votes
0
replies
95
views
How label Group list after integratin scRNA-seq and scATAC-seq data
ArchR
1 day ago by
Bioinformatics_16
• 0
0
votes
1
reply
160
views
Forum:
Questions on what to focus on in the next 3-4 years of my research
Career
offtopic
path
11 hours ago by
Abiodun
• 0
0
votes
7
replies
409
views
Error in importing data from 10x genomics into RStudio
H5
file
scRNA
RStudio
16 hours ago by
himanshu0102
• 0
1
vote
2
replies
198
views
WGS for CNV and Aneuploidy analysis
CNV
Aneuploidy
WGS
7 hours ago by
bioinfo1990
▴ 10
0
votes
0
replies
108
views
News:
Course: Phylogenetic Comparative Methods in R – May 12–16 (Online)
R
Phylogenetic-Comparative-Methods
Ultrametric-Phylogenies
updated 15 hours ago by
Ram
45k • written 2 days ago by
Physalia-courses
★ 2.6k
2
votes
2
replies
239
views
Merge and unique multiple complete sam files from identical fastq files
samtools
aligment
sambamba
WGBS
WGS
1 day ago by
JustinZhang
▴ 120
0
votes
1
reply
509
views
Issues with T2T in pigeon prepare from isoseq
long-read
isoseq
rna-seq
updated 15 hours ago by
Ram
45k • written 6 months ago by
SethJ
• 0
1
vote
1
reply
304
views
How to obtain TPM data after batch effect correction from count data obtained using RSEM
STAR
RNA-seq
Batch
RSEM
effect
updated 3 days ago by
Gordon Smyth
★ 7.9k • written 3 days ago by
Apprentice
▴ 170
1
vote
1
reply
351
views
Missing short indels from vcf
bcftools
shortindels
mpileup
updated 3 days ago by
LauferVA
4.7k • written 6 days ago by
kbranger
• 0
4
votes
6
replies
529
views
Facing issue with output of nextflow pipeline
output
nextflow
fastqc
issue
updated 3 days ago by
mmhryc
• 0 • written 6 days ago by
harsh
▴ 20
1
vote
2
replies
281
views
Choosing the best clustering method for a certain methylation data
DMR
k-means
hierarchical
clustering
methylation
updated 3 days ago by
5heikki
11k • written 4 days ago by
Sophia
• 0
4
votes
7
replies
487
views
Extract length of sample sequence from vcf
microsatellites
bcftools
vcf
indels
variants
updated 50 minutes ago by
Istvan Albert
102k • written 4 days ago by
jahnreinhard.ringger
• 0
1
vote
14
replies
613
views
MultiQC_report
adapter
Quality-control
fastqc
multiqc
updated 30 minutes ago by
GenoMax
150k • written 4 days ago by
AIMAR
• 0
4
votes
3
replies
328
views
Forum:
How to get into research for an absolute beginners
research
updated 1 day ago by
Yugan Gogul Muthukumar
▴ 10 • written 4 days ago by
jpeduprep
• 0
0
votes
0
replies
182
views
News:
Online course — Comparative Genomics — 7–11 April
SNVs
ComparativeGenomics
SVs
GenomeAnnotation
4 days ago by
Physalia-courses
★ 2.6k
0
votes
2
replies
269
views
how to do a corellation analysis of multiple CRISPR data sets
crispr-screen
batch-effect
correlation
updated 15 hours ago by
Ram
45k • written 4 days ago by
Assa Yeroslaviz
★ 1.9k
8
votes
4
replies
505
views
Alternatives to UCSC genome browser for obtaining gene coordinates
browser
ucsc
updated 4 days ago by
LauferVA
4.7k • written 6 days ago by
shpak.max
▴ 60
1
vote
12
replies
676
views
Tool:
Introducing Grantease: Go From RFA to First Draft Grant Proposal in 15 Minutes
ai
biology
grants
nih
litreview
updated 21 hours ago by
jared.andrews07
★ 18k • written 4 days ago by
Will
• 0
0
votes
0
replies
221
views
Job:
Postdoctoral Position in Computational Protein Design and Molecular Modelling
protein-design
molecular-modeling
postdoc
updated 15 hours ago by
Ram
45k • written 4 days ago by
Sophie
• 0
1
vote
3
replies
382
views
Modify read groups in BAM file
samtools
BAM
picard
DRAGEN
updated 3 days ago by
1769mkc
★ 1.2k • written 4 days ago by
Alex
• 0
0
votes
1
reply
264
views
How to put distinct colors to connection bridges in the Sankey plot ?
R
Python
Sankey
updated 4 days ago by
zx8754
12k • written 4 days ago by
ohtang7
▴ 40
1
vote
1
reply
264
views
PCA comparison of cell line vs tumors
RNAseq
Sequencing
PCA
Normalization
updated 1 day ago by
ATpoint
87k • written 5 days ago by
karlensberg
• 0
2
votes
9
replies
585
views
Find the reads which correspond to DP of vcf
bam
variant
vcf
sam2tsv
updated 5 days ago by
Pierre Lindenbaum
165k • written 6 days ago by
totoroGirl
• 0
120,922 results • Page
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Answer: Getting sequences fragments using pysam
C: Does EnhancedVolcano() supports crosstalk()?
C: Does EnhancedVolcano() supports crosstalk()?
A: What Does The Sequence Nnnn... Mean On A Sam File?
A: What Does The Sequence Nnnn... Mean On A Sam File?
Answer: Question About Handling Technical Replicates in ATAC-seq
Comment: Polishing raw nanopore reads with Pacbio
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Recent Replies
Comment: C.reinhardtii single-cell Seurat object - gene names instead of loci
by
Ana
• 0
Thank you for your answer! Unfortunalety, NCBI doesn't have the latest version of the genome and annotations for this organism (v6.1), so I…
Comment: Has anybody worked with C.reinhardtii single-cell before and could help me get t
by
GenoMax
150k
It is always safer to get sequence/annotations from standard sources like NCBI/Ensembl so no manipulations are required to make the data wo…
Comment: Snakemake Global Environment Container
by
DBScan
▴ 470
How are you running snakemake?
Comment: MultiQC_report
by
GenoMax
150k
Moved this to an answer since it seems to be a logical explanation for the observation reported in original post.
Comment: Poor alignment rates using Bowtie
by
GenoMax
150k
Looking at the size distribution the data must already be pre-trimmed so it does not make sense to trim again. You should merge data from t…
Answer: MultiQC_report
by
Basti
★ 2.0k
I think it just counts the total number of lines while there could be multiple adapters types per sample (ex : illumina_universal_adapter, …
Comment: Poor alignment rates using Bowtie
by
Istvan Albert
102k
If your reference has invalid bases then you can get quite unexpected behaviors. I am not sure what takes place - probably the scoring is…
Comment: Is there a tool to obtain GO terms for thousands of genes at once?
by
Ana
• 0
I got it figured out, thanks!
Comment: Polishing raw nanopore reads with Pacbio
by
GenoMax
150k
Since only tools available (confirmed by @colindaven ) require illumina reads you would be basically creating "illumina like" reads from yo…
Comment: Extract length of sample sequence from vcf
by
Istvan Albert
102k
I would use a tool like cyvcf2 https://brentp.github.io/cyvcf2/ It provides a simple pythonic interface to VCF data. You can then easily …
Comment: Polishing raw nanopore reads with Pacbio
by
samuel.a.odonnell
▴ 590
As in fragmenting (and pairing) the hifi reads? Do you think this would have any improvement over actual illumina reads?
Comment: Polishing raw nanopore reads with Pacbio
by
samuel.a.odonnell
▴ 590
Thanks for confirming the absence of a tool, I think I can give up the hunt now. I've often done it this typical way of polishing after; ho…
Comment: About the Tool FASTP
by
shelkmike
★ 1.5k
One problem that I encounter if I don't trim reads is that taxonomic classification of untrimmed reads often produces incorrect results. Th…
Comment: PopGenome lost individual information
by
Arup Ghosh
3.3k
Can you share the command you are using?
Comment: Discrepancy between positions on IGV and NCBI's PrimerBLAST
by
Rozita
▴ 40
It showed me the same positions as that of PrimerBLAST. Any idea how do I now visualise the publicly available datasets that I have and co…
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