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121,005 results • Page
1 of 2421
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Rank
Views
Votes
Replies
0
votes
4
replies
197
views
UCSC's NCBI RefSeq Track tables: header differences
ucsc-genome-browswer
refseq
ncbi
7 hours ago by
Synanth
• 0
2
votes
2
replies
1.2k
views
How do you do differential splicing using edgeR command diffSpliceDGE?
differential-splicing
diffSpliceDGE
edger
updated 1 hour ago by
Gordon Smyth
★ 7.9k • written 3.2 years ago by
Assa Yeroslaviz
★ 1.9k
0
votes
0
replies
68
views
Any recommendation for calculating Fu and Li's D using RStudio?
PopGenome
updated 11 hours ago by
GenoMax
150k • written 15 hours ago by
Chatchapon
• 0
836
votes
167
replies
173k
views
111 follow
News:
The Biostar Handbook. A bioinformatics e-book for beginners.
training
handbook
updated 5 days ago by
Biostar
3.5k • written 8.4 years ago by
Istvan Albert
102k
0
votes
0
replies
67
views
sequenced noise elimination in searching for snp
snp
sequencing
virus
16 hours ago by
gagi1993
• 0
0
votes
1
reply
122
views
Issue with Agilent 2100 BioAnalyzer HS DNA Assay Trace
library-preparation
DNA
bioanalyzer
updated 11 hours ago by
GenoMax
150k • written 1 day ago by
Beatrice
• 0
1
vote
2
replies
658
views
QIAseq 16S/ITS Screening Panel Primers?
16s
primers
metagenomics
ITS
updated 1 day ago by
GenoMax
150k • written 3.2 years ago by
Isaac
• 0
1
vote
1
reply
172
views
Analysing gene CNV from TCGA using TCGAbiolinks
TCGA
updated 1 day ago by
Zhenyu Zhang
★ 1.3k • written 2 days ago by
Matt
• 0
1
vote
3
replies
226
views
How to use LOEUF plugin in VEP?
LOEUF
VEP
updated 1 day ago by
GenoMax
150k • written 1 day ago by
Sd
• 0
0
votes
0
replies
155
views
Trouble with CIBERSORTx: All P-values > 0.05, Low Correlation, High RMSE – What Could Be Going Wrong?
R
CIBERSORTx
CIBERSORT
script
updated 1 day ago by
GenoMax
150k • written 1 day ago by
Pumla
• 0
0
votes
1
reply
122
views
How can I identify genes with the MSTRG notation?
R
stringtie
RNA-Seq
updated 1 day ago by
GenoMax
150k • written 1 day ago by
pinheirofabiano
▴ 110
0
votes
0
replies
396
views
dittoHeapmap didn't work in my data set(error code :Error in .subscript.2ary(x, i, j, drop = TRUE) : subscript out of bounds)
dittoseq
scRNAseq
updated 1 day ago by
ATpoint
87k • written 1 day ago by
kayah
▴ 20
1
vote
3
replies
193
views
Visualise duplications and inversions
inversions
duplications
updated 8 hours ago by
cmdcolin
★ 4.2k • written 1 day ago by
priya.bmg
▴ 70
1
vote
1
reply
144
views
Exon-level counts via featureCounts
diffSpliceDGE
exon
RNASeq
featureCounts
edgeR
updated 1 day ago by
GenoMax
150k • written 1 day ago by
n_navy
• 0
0
votes
5
replies
229
views
Picard : Bait_interval and Target_interval
Picard
Bed
read-depth
coverage
updated 1 day ago by
Ram
45k • written 1 day ago by
AIMAR
• 0
3
votes
2
replies
177
views
Does vg giraffe output secondary and supplimentary alignments?
vg
giraffe
1 day ago by
lushjia
• 0
0
votes
0
replies
124
views
Question about Calculation of Dietary index in the NHANES Database
index
DI-GM
Dietary
NHANES
2 days ago by
城玮
• 0
0
votes
1
reply
212
views
Detection SNPs and Indels Bacteria
SNPs
updated 2 days ago by
Istvan Albert
102k • written 2 days ago by
Eltercertopico
• 0
1
vote
4
replies
387
views
protease candidates for my protein of interest
cleaving-site
proteases
protein
2 days ago by
Fatemeh
▴ 40
1
vote
3
replies
250
views
Different number of fragments after doublets removal in each run
ArchR
22 hours ago by
Bioinformatics_16
• 0
1
vote
2
replies
204
views
Seeking Guidance on Next Steps for DNA Methylation Biomarker Screening in Cancer Prognosis
early-cancer-screening
TCGA
Epigenomics
DNA-Methylation
cancer-prognosis
15 hours ago by
Riley J
• 0
0
votes
1
reply
190
views
Help with Panel of Normal (PON)
somatic-mutation
PoN
updated 2 days ago by
Ram
45k • written 2 days ago by
TJ
• 0
7
votes
3
replies
342
views
Why don't I see people calculating FPKM from normalized read counts?
normalization
RNA-seq
updated 1 day ago by
kalavattam
▴ 350 • written 3 days ago by
shelkmike
★ 1.5k
1
vote
9
replies
516
views
Error during running wf-bacterial-genome (nextflow run epi2me-labs/wf-bacterial-genomes).
EPI2ME
MEDAKA
updated 11 hours ago by
GenoMax
150k • written 3 days ago by
Anurag
• 0
0
votes
0
replies
126
views
freebayes with pools of different number of individuals
freebayes
poolseq
2 days ago by
Jeanlain
• 0
0
votes
3
replies
240
views
Insight into Trimmomatic
Trimmomatic
Trimming
Reads
updated 2 days ago by
GenoMax
150k • written 3 days ago by
jh5ym
• 0
1
vote
3
replies
269
views
Trouble when uploading FASTQ files on NCBI
rnaseq
ncbi
1 day ago by
Gerard
▴ 20
1
vote
4
replies
296
views
SyntaxError in file /snakefile, line 22: invalid decimal literal: None
python
snakemake
updated 2 days ago by
Jesse
▴ 870 • written 3 days ago by
Ana
▴ 10
3
votes
2
replies
299
views
News:
Building an mmCIF parser for enzyme structure analysis — feedback & ideas welcome!
mmcif
3 days ago by
Shravya
• 0
0
votes
0
replies
163
views
News:
Course: Introduction to Genome Annotation (Online, 19–22 May 2025)
Gene-Prediction
Genome-Annotation
Gene-Models
3 days ago by
Physalia-courses
★ 2.6k
0
votes
1
reply
198
views
Error with running cellranger-atac count
cellranger
atac
single-cell
updated 3 days ago by
Ram
45k • written 3 days ago by
Khôi
• 0
0
votes
2
replies
267
views
HIC-pro with Forward and reverse reads not paired even when I use their test data.
HIC-pro
2 days ago by
Aki
▴ 20
1
vote
1
reply
371
views
Using DEXseq on kallisto tsv outputs ?
alignement
DEXseq
kallisto
updated 4 days ago by
Gordon Smyth
★ 7.9k • written 9 days ago by
Gerard
▴ 20
0
votes
0
replies
214
views
Job:
2025 Arrowhead AI/LLM for Computational Biology and Data Science Internship
intern
updated 4 days ago by
GenoMax
150k • written 4 days ago by
Shicheng Guo
★ 9.6k
1
vote
2
replies
565
views
ChIP-seq gene blacklist for Rattus norvegicus
rat
blacklist
ChIP-seq
updated 4 days ago by
GenoMax
150k • written 12 months ago by
Thomas
• 0
4
votes
10
replies
907
views
6 follow
Extract length of sample sequence from vcf
microsatellites
bcftools
vcf
indels
variants
updated 4 days ago by
cmdcolin
★ 4.2k • written 14 days ago by
jahnreinhard.ringger
• 0
0
votes
0
replies
196
views
Tutorial:
Fast SCENIC GRN Analysis with RegDiffusion
GRN
network
scRNA-seq
wgcna
4 days ago by
Hao
▴ 10
2
votes
3
replies
693
views
Is it reasonable to do "Gene Regulatory Network Inference" for each cell type and do differential GRN
GRN
scRNA-seq
updated 4 days ago by
Hao
▴ 10 • written 2.6 years ago by
shangguandong1996
▴ 30
1
vote
2
replies
444
views
Blastx issue
blastx
lncRNA
updated 3 days ago by
Ram
45k • written 7 days ago by
Avik
• 0
2
votes
9
replies
653
views
TPM vs FPKM divergence at high values
tpm
fpkm
normalisation
updated 3 days ago by
Istvan Albert
102k • written 6 days ago by
megancgrdnr
• 0
3
votes
0
replies
216
views
Herald:
The Biostar Herald for Monday, April 14, 2025
herald
4 days ago by
Biostar
3.5k
1
vote
2
replies
367
views
What is the best way to know if a pair of genes is random?
go-terms
genes
updated 3 days ago by
Ram
45k • written 7 days ago by
Ana
▴ 10
1
vote
1
reply
303
views
How to save the AlphaFold PAE plot as an image in ChimeraX using command line ?
PAE
ChimeraX
AlphaFold
updated 4 days ago by
LauferVA
4.7k • written 6 days ago by
Picasa
▴ 680
1
vote
0
replies
185
views
News:
LAST CALL - Bioinformatics Pipeline Development with Nextflow - May 19-21, 2025 (online) - Apply Now
nextflow
rnaseq
workshop
4 days ago by
ecSeq Bioinformatics
▴ 20
1
vote
2
replies
321
views
RNA-seq, three comparisons - stick to one linear model, or use separate models?
statistics
mixed-effect-model
stats
RNAseq
linear-model
updated 3 days ago by
Ram
45k • written 5 days ago by
noodlejackson
▴ 40
1
vote
1
reply
247
views
Using Principal Components may muddle the detecting power of a GWAS?
q-value
covariates
genomic-inflation
pca
gwas
updated 4 days ago by
DBScan
▴ 480 • written 4 days ago by
AndrMod
• 0
0
votes
0
replies
197
views
Use Seed or Full Alignments from Pfam HMM as a filter step
hmm
sequences
metagenomic
4 days ago by
Janelyn123
• 0
3
votes
4
replies
388
views
Helicobacter pylori low-rate alignment
RNA-seq
transcriptomics
updated 3 days ago by
Ram
45k • written 5 days ago by
Ariadna
▴ 20
14
votes
3
replies
11k
views
Can The Wget Command Be Used To Download Pdbs
pdb
updated 4 days ago by
Calvin
• 0 • written 13.5 years ago by
Harpalss
▴ 310
0
votes
3
replies
408
views
Is a log2 transformation an essential step in preparing expression data for machine learning?
Machine-learning
preproccessing
microarray
updated 3 days ago by
Ram
45k • written 5 days ago by
Sib
▴ 60
121,005 results • Page
1 of 2421
Recent Votes
A: FindConservedMarkers vs FindMarkers vs FindAllMarkers Seurat
Answer: Missing short indels from vcf
Answer: Analysing gene CNV from TCGA using TCGAbiolinks
Comment: Visualise duplications and inversions
Answer: How do you do differential splicing using edgeR command diffSpliceDGE?
Answer: Why don't I see people calculating FPKM from normalized read counts?
Comment: Why don't I see people calculating FPKM from normalized read counts?
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Maximilian Haeussler
★ 1.7k
Popular Question
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kuttibiotech2009
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kb_93
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Recent Replies
Comment: why I get "Running twoBitToFa failed" when loading chopchop
by
GenoMax
150k
Good to know.
Comment: why I get "Running twoBitToFa failed" when loading chopchop
by
Eric
• 0
My issue was I didn't have full paths in my JSON "PATH": { "PRIMER3": "/path/to/chopchop/primer3_core", "BOWTIE": "/…
Comment: why I get "Running twoBitToFa failed" when loading chopchop
by
Eric
• 0
This is the command: /chopchop_query.py \ --genePred_file "/path/to/Zmays.gene_table" \ -G "Zmays" \ -o "${OUTPUT}" …
Comment: Short reads to identify stuctural variants
by
priya.bmg
▴ 70
These insertions are seen in tandem repeat regions, which could have lead to the poor alignment in the flanking regions. CIGAR also showed …
Comment: Short reads to identify stuctural variants
by
priya.bmg
▴ 70
Yes, I am working in families. We did whole genome long read sequencing for three individuals in each family. To confirm if these structura…
Comment: why I get "Running twoBitToFa failed" when loading chopchop
by
GenoMax
150k
Can you clarify what step you are getting the error at and the command you are running? Original poster was not clear about that either.
Comment: why I get "Running twoBitToFa failed" when loading chopchop
by
Eric
• 0
Did you get this fixed?
Comment: UCSC's NCBI RefSeq Track tables: header differences
by
Synanth
• 0
It's transposable element quantification software. I don't think bin is necessary due to some of the authors responses to issues n github. …
Comment: UCSC's NCBI RefSeq Track tables: header differences
by
Synanth
• 0
Thank you for the resources.
Comment: Visualise duplications and inversions
by
cmdcolin
★ 4.2k
SVbyEye would probably be used in the case that you have e.g. a de-novo genome assembly to compare to the reference. it isn't built for vis…
Comment: Issue with Agilent 2100 BioAnalyzer HS DNA Assay Trace
by
GenoMax
150k
Cross-posted to SeqAnswers: https://www.seqanswers.com/forum/applications-forums/sample-prep-library-generation/326545-issue-with-agilent-2…
Comment: Error during running wf-bacterial-genome (nextflow run epi2me-labs/wf-bacterial-
by
GenoMax
150k
If you currently have only FAST basecalls and would like to get the higher quality HAC/SUP calls then you have to use `dorado`. It may be…
Answer: UCSC's NCBI RefSeq Track tables: header differences
by
Maximilian Haeussler
★ 1.7k
What is this software? Most likely, it will just ignore the "bin" column, you can put 0 into that, I bet. As for the other columns, I thin…
Comment: Error during running wf-bacterial-genome (nextflow run epi2me-labs/wf-bacterial-
by
Anurag
• 0
I am using EPI2ME nextflow beacterial genome pipeline is it still necessary to use dorado basecaller first ?
Comment: Seeking Guidance on Next Steps for DNA Methylation Biomarker Screening in Cancer
by
Riley J
• 0
Dear LauferVA, Thank you for your sincere advice. You are right, and I fully agree with you. I have just started to get involved in bioinf…
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