Latest
Open
Jobs
Tutorials
Tags
About
FAQ
Community
Planet
New Post
Log In
New Post
Latest
Open
News
Jobs
Tutorials
Forum
Tags
Planet
Users
Log In
Sign Up
About
Post does not exist.
Limit : all time
all time
today
this week
this month
this year
120,987 results • Page
1 of 2420
Sort: Rank
Rank
Views
Votes
Replies
0
votes
1
reply
38
views
Insight into Trimmomatic
Trimmomatic
Trimming
Reads
updated 2 hours ago by
GenoMax
150k • written 3 hours ago by
jh5ym
• 0
0
votes
2
replies
69
views
Error during running wf-bacterial-genome (nextflow run epi2me-labs/wf-bacterial-genomes).
EPI2ME
MEDAKA
updated 2 hours ago by
Ram
45k • written 4 hours ago by
Anurag
• 0
1
vote
1
reply
82
views
Why don't I see people calculating FPKM from normalized read counts?
normalization
RNA-seq
updated 3 hours ago by
Istvan Albert
102k • written 7 hours ago by
shelkmike
★ 1.5k
0
votes
2
replies
90
views
Trouble when uploading FASTQ files on NCBI
rnaseq
ncbi
updated 3 hours ago by
Ram
45k • written 7 hours ago by
Gerard
▴ 10
0
votes
2
replies
94
views
SyntaxError in file /snakefile, line 22: invalid decimal literal: None
python
snakemake
updated 3 hours ago by
Ram
45k • written 8 hours ago by
Ana
• 0
836
votes
167
replies
173k
views
111 follow
News:
The Biostar Handbook. A bioinformatics e-book for beginners.
training
handbook
updated 2 days ago by
Biostar
3.5k • written 8.4 years ago by
Istvan Albert
102k
3
votes
2
replies
165
views
News:
Building an mmCIF parser for enzyme structure analysis — feedback & ideas welcome!
mmcif
6 hours ago by
Shravya
• 0
0
votes
0
replies
71
views
News:
Course: Introduction to Genome Annotation (Online, 19–22 May 2025)
Gene-Prediction
Genome-Annotation
Gene-Models
14 hours ago by
Physalia-courses
★ 2.6k
0
votes
1
reply
98
views
Error with running cellranger-atac count
cellranger
atac
single-cell
updated 3 hours ago by
Ram
45k • written 15 hours ago by
Khôi
• 0
0
votes
1
reply
109
views
HIC-pro with Forward and reverse reads not paired even when I use their test data.
HIC-pro
updated 9 hours ago by
GenoMax
150k • written 22 hours ago by
Aki
▴ 20
0
votes
1
reply
271
views
Using DEXseq on kallisto tsv outputs ?
alignement
DEXseq
kallisto
updated 1 day ago by
Gordon Smyth
★ 7.9k • written 6 days ago by
Gerard
▴ 10
0
votes
0
replies
103
views
Job:
2025 Arrowhead AI/LLM for Computational Biology and Data Science Internship
intern
updated 1 day ago by
GenoMax
150k • written 1 day ago by
Shicheng Guo
★ 9.6k
1
vote
2
replies
469
views
ChIP-seq gene blacklist for Rattus norvegicus
rat
blacklist
ChIP-seq
updated 1 day ago by
GenoMax
150k • written 12 months ago by
Thomas
• 0
4
votes
10
replies
779
views
6 follow
Extract length of sample sequence from vcf
microsatellites
bcftools
vcf
indels
variants
updated 1 day ago by
cmdcolin
★ 4.2k • written 11 days ago by
jahnreinhard.ringger
• 0
0
votes
0
replies
100
views
Tutorial:
Fast SCENIC GRN Analysis with RegDiffusion
GRN
network
scRNA-seq
wgcna
1 day ago by
Hao
▴ 10
2
votes
3
replies
543
views
Is it reasonable to do "Gene Regulatory Network Inference" for each cell type and do differential GRN
GRN
scRNA-seq
updated 1 day ago by
Hao
▴ 10 • written 2.6 years ago by
shangguandong1996
▴ 30
1
vote
2
replies
299
views
Blastx issue
blastx
lncRNA
updated 2 hours ago by
Ram
45k • written 4 days ago by
Avik
• 0
2
votes
9
replies
499
views
TPM vs FPKM divergence at high values
tpm
fpkm
normalisation
updated 7 hours ago by
Istvan Albert
102k • written 3 days ago by
megancgrdnr
• 0
3
votes
0
replies
116
views
Herald:
The Biostar Herald for Monday, April 14, 2025
herald
1 day ago by
Biostar
3.5k
0
votes
2
replies
265
views
What is the best way to know if a pair of genes is random?
go-terms
genes
updated 2 hours ago by
Ram
45k • written 4 days ago by
Ana
• 0
1
vote
1
reply
209
views
How to save the AlphaFold PAE plot as an image in ChimeraX using command line ?
PAE
ChimeraX
AlphaFold
updated 1 day ago by
LauferVA
4.7k • written 3 days ago by
Picasa
▴ 680
1
vote
0
replies
91
views
News:
LAST CALL - Bioinformatics Pipeline Development with Nextflow - May 19-21, 2025 (online) - Apply Now
nextflow
rnaseq
workshop
1 day ago by
ecSeq Bioinformatics
▴ 20
1
vote
2
replies
220
views
RNA-seq, three comparisons - stick to one linear model, or use separate models?
statistics
mixed-effect-model
stats
RNAseq
linear-model
updated 2 hours ago by
Ram
45k • written 2 days ago by
noodlejackson
▴ 40
1
vote
1
reply
136
views
Using Principal Components may muddle the detecting power of a GWAS?
q-value
covariates
genomic-inflation
pca
gwas
updated 1 day ago by
DBScan
▴ 480 • written 1 day ago by
AndrMod
• 0
0
votes
0
replies
106
views
Use Seed or Full Alignments from Pfam HMM as a filter step
hmm
sequences
metagenomic
1 day ago by
Janelyn123
• 0
3
votes
4
replies
272
views
Helicobacter pylori low-rate alignment
RNA-seq
transcriptomics
updated 2 hours ago by
Ram
45k • written 2 days ago by
Ariadna
▴ 20
14
votes
3
replies
11k
views
Can The Wget Command Be Used To Download Pdbs
pdb
updated 1 day ago by
Calvin
• 0 • written 13.5 years ago by
Harpalss
▴ 310
0
votes
3
replies
292
views
Is a log2 transformation an essential step in preparing expression data for machine learning?
Machine-learning
preproccessing
microarray
updated 2 hours ago by
Ram
45k • written 2 days ago by
Sib
▴ 60
0
votes
2
replies
878
views
How to re-annotate the structure of gene against a known genomic file
RNA-Seq
updated 2 days ago by
Mensur Dlakic
★ 29k • written 5.9 years ago by
Kanoo
• 0
0
votes
2
replies
247
views
ONT Live-Basecalling Issue?
ONT
minKNOW
live-basecalling
updated 16 hours ago by
LauferVA
4.7k • written 3 days ago by
Akshay
• 0
3
votes
4
replies
292
views
Identify gene from sequence at scale in non-model organisms
sequence
gene
updated 2 hours ago by
Ram
45k • written 3 days ago by
matthewmerkin32
• 0
0
votes
0
replies
152
views
News:
Cellosaurus release 52 is available
biocuration
database
knowledgebase
cell-line
updated 2 hours ago by
Ram
45k • written 3 days ago by
Amos Bairoch
▴ 130
0
votes
2
replies
284
views
WGCNA
WGCNA
updated 2 hours ago by
Ram
45k • written 4 days ago by
香
• 0
0
votes
0
replies
290
views
Seeking Advice on Causal Inference for Treatment Effect Prediction (Small Sample, Genomic Covariates)
causal-inference
updated 2 hours ago by
Ram
45k • written 3 days ago by
oghzzang
▴ 50
5
votes
5
replies
457
views
Batch effect
RNAseq
Batch-Effect
2 days ago by
Fatemeh
▴ 40
0
votes
4
replies
392
views
convert Gene ID to Gene Symbol
gene-id
updated 3 days ago by
pinheirofabiano
▴ 110 • written 6 days ago by
majorprogeria
• 0
0
votes
1
reply
220
views
News:
Structural bioinformatics workshop
Structural-bioinformatics
updated 2 hours ago by
Ram
45k • written 4 days ago by
reetesh
• 0
1
vote
10
replies
586
views
How to test the similarity of two gene expression profiles
gene-sets
updated 2 hours ago by
Ram
45k • written 5 days ago by
htchd
• 0
3
votes
3
replies
256
views
Which are the different kinds of GeneID that exist?
R
RNA-Seq
4 days ago by
pinheirofabiano
▴ 110
0
votes
0
replies
168
views
Error using DWLS
deconvolution
DWLS
RNAseq
4 days ago by
Ana
• 0
0
votes
5
replies
1.7k
views
How to remove this module selection error?
WGCNA
updated 4 days ago by
香
• 0 • written 2.3 years ago by
gupta00aritra
• 0
0
votes
1
reply
1.0k
views
bbsplit : How to configure ambiguous/ambiguous2 parameters in order to consider as unmapped reads, reads mapped on both human/mouse references.
bbsplit
nextflow
updated 4 days ago by
GenoMax
150k • written 4 days ago by
ZheFrench
▴ 590
1
vote
2
replies
245
views
Limma for DEG analysis
R
DEG
limma
updated 2 hours ago by
Ram
45k • written 4 days ago by
rahma.khoualdia
• 0
0
votes
0
replies
212
views
deletion impact
vcf
updated 4 days ago by
GenoMax
150k • written 4 days ago by
HarperReed
• 0
0
votes
2
replies
265
views
Help finding GTEx v8 file
GTEx
SHAPEIT2
3 days ago by
蒲蒲
• 0
2
votes
5
replies
313
views
Idat file processing issue with command line cli
CanineHTS
IDAT
4 days ago by
1769mkc
★ 1.2k
0
votes
0
replies
173
views
is there a way to get --ref paths to prioritize my order with vg call?
call
vg
vgcall
22 hours ago by
PolenP
• 0
0
votes
1
reply
234
views
Has anyone tried used simple ML models to identify virulence genes?
genetics
R
classifier
genomics
machinelearning
updated 4 days ago by
Mensur Dlakic
★ 29k • written 4 days ago by
Jack
• 0
0
votes
4
replies
354
views
What does vg autoindex mpmap do?
vg
mpmap
autoindex
1 day ago by
Ziyue
• 0
5
votes
4
replies
404
views
How to keep the same view for different AlphaFold 3 simulations in ChimeraX?
chimerax
alphafold
3
updated 4 days ago by
LauferVA
4.7k • written 5 days ago by
Picasa
▴ 680
120,987 results • Page
1 of 2420
Recent Votes
Counting number of reads and bases fastQ with python
Answer: Whole transcriptome analysis nominated primarily miRNA-related pathways. Advice?
Comment: Using Principal Components may muddle the detecting power of a GWAS?
Answer: Building an mmCIF parser for enzyme structure analysis — feedback & ideas welcom
Why don't I see people calculating FPKM from normalized read counts?
Comment: makeblastdb Bus error
Answer: Building an mmCIF parser for enzyme structure analysis — feedback & ideas welcom
Recent Locations •
All
Australia,
3 minutes ago
United States,
15 minutes ago
United States,
22 minutes ago
United States,
22 minutes ago
University of Hamburg,
27 minutes ago
Canada,
33 minutes ago
United States,
46 minutes ago
Recent Awards •
All
Popular Question
to
LauferVA
4.7k
Popular Question
to
Jaykumar
▴ 50
Scholar
to
ATpoint
87k
Popular Question
to
theodore
▴ 110
Popular Question
to
Assa Yeroslaviz
★ 1.9k
Popular Question
to
oghzzang
▴ 50
Popular Question
to
Shicheng Guo
★ 9.6k
Recent Replies
Comment: Insight into Trimmomatic
by
GenoMax
150k
It would definitely be useful to first run FastQC/MultiQC on your data to understand the overall characteristics of the data. Make a note o…
Comment: Error during running wf-bacterial-genome (nextflow run epi2me-labs/wf-bacterial-
by
Anurag
• 0
hello I am very new to this field, I am trying to assemble genome using wf-bacterial-genome (nextflow run epi2me-labs/wf-bacterial-genomes…
Comment: Error during running wf-bacterial-genome (nextflow run epi2me-labs/wf-bacterial-
by
GenoMax
150k
Please add some detail about the command you are running and what exactly you are trying to do. You may have put part of the command line i…
Comment: Building an mmCIF parser for enzyme structure analysis — feedback & ideas welcom
by
Shravya
• 0
Thank you so much for the clarification! You're right — I should've phrased it more as building a parser "for mmCIF files" in general, and…
Comment: Why don't I see people calculating FPKM from normalized read counts?
by
Istvan Albert
102k
Scientists stopped using FPKM in general because better measures are available: https://www.biostars.org/p/104214/#332357 > *The Total Co…
Comment: Trouble when uploading FASTQ files on NCBI
by
lieven.sterck
15k
Not solving this particular problem but merely a suggestion: Given your 'location' you might also consider uploading data to ENA / arrayEx…
Comment: SyntaxError in file /snakefile, line 22: invalid decimal literal: None
by
Jesse
▴ 860
That's [Python's f-string](https://docs.python.org/3/tutorial/inputoutput.html#formatted-string-literals), which in this case just turns `{…
Comment: TPM vs FPKM divergence at high values
by
Istvan Albert
102k
I admit I had no firm idea what a TPM vs FPKM plot might look like. I never computed both, but this motivated me enough to take a salmon …
Comment: Trouble when uploading FASTQ files on NCBI
by
GenoMax
150k
This may be hard to help/diagnose for biostar community since we don't know about your local set up. Problem could be on either end (your o…
Comment: TPM vs FPKM divergence at high values
by
dariober
15k
Yes, the axes are both logarithmic, the difference is in the input data with FPKM being log'd and TPM being raw.
Comment: SyntaxError in file /snakefile, line 22: invalid decimal literal: None
by
ATpoint
87k
I am not a SnakeMake person but what is the `f` supposed to be? `f'/{{...`
Comment: TPM vs FPKM divergence at high values
by
Istvan Albert
102k
I think both are logarithmic axes. Just one is in exponential notation the other is not.
Comment: HIC-pro with Forward and reverse reads not paired even when I use their test dat
by
GenoMax
150k
Looks like the original dataset has an equal number of records based on the lines so that input looks correct. $ wc -l SRR400264_* …
Comment: MULTIQC for miRNAseq data
by
Alana Conceição Maia Lessa
• 0
Thank you so much!
Comment: Error with running cellranger-atac count
by
GenoMax
150k
> Before processing the fastq files with the count command, I was trimming primer & linker sequences according to a paper I was looking at.…
Traffic: 2009 users visited in the last hour
Content
Search
Users
Tags
Badges
Help
About
FAQ
Access
RSS
API
Stats
Use of this site constitutes acceptance of our
User Agreement and Privacy Policy
.
Powered by the
version 2.3.6