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120,980 results • Page
1 of 2420
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Votes
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0
votes
0
replies
36
views
HIC-pro with Forward and reverse reads not paired even when I use their test data.
HIC-pro
3 hours ago by
Aki
▴ 20
0
votes
1
reply
211
views
Using DEXseq on kallisto tsv outputs ?
alignement
DEXseq
kallisto
updated 4 hours ago by
Gordon Smyth
★ 7.9k • written 5 days ago by
Gerard
▴ 10
0
votes
0
replies
52
views
Job:
2025 Arrowhead AI/LLM for Computational Biology and Data Science Internship
intern
updated 7 hours ago by
GenoMax
150k • written 7 hours ago by
Shicheng Guo
★ 9.6k
1
vote
2
replies
427
views
ChIP-seq gene blacklist for Rattus norvegicus
rat
blacklist
ChIP-seq
updated 8 hours ago by
GenoMax
150k • written 12 months ago by
Thomas
• 0
4
votes
10
replies
736
views
6 follow
Extract length of sample sequence from vcf
microsatellites
bcftools
vcf
indels
variants
updated 9 hours ago by
cmdcolin
★ 4.2k • written 10 days ago by
jahnreinhard.ringger
• 0
836
votes
167
replies
173k
views
111 follow
News:
The Biostar Handbook. A bioinformatics e-book for beginners.
training
handbook
updated 1 day ago by
Biostar
3.4k • written 8.4 years ago by
Istvan Albert
102k
0
votes
0
replies
63
views
Tutorial:
Fast SCENIC GRN Analysis with RegDiffusion
GRN
network
scRNA-seq
wgcna
11 hours ago by
Hao
▴ 10
2
votes
3
replies
481
views
Is it reasonable to do "Gene Regulatory Network Inference" for each cell type and do differential GRN
GRN
scRNA-seq
updated 11 hours ago by
Hao
▴ 10 • written 2.6 years ago by
shangguandong1996
▴ 30
1
vote
2
replies
255
views
Blastx issue
Long
coding
non
blastx
RNA
updated 11 hours ago by
colindaven
7.4k • written 3 days ago by
Avik
• 0
0
votes
5
replies
337
views
TPM vs FPKM divergence at high values
tpm
fpkm
normalisation
updated 12 hours ago by
Istvan Albert
102k • written 2 days ago by
megancgrdnr
• 0
2
votes
0
replies
71
views
Herald:
The Biostar Herald for Monday, April 14, 2025
herald
13 hours ago by
Biostar
3.4k
0
votes
1
reply
199
views
What is the best way to know if a pair of genes is random?
terms
genes
go
updated 13 hours ago by
shelkmike
★ 1.5k • written 3 days ago by
Ana
• 0
0
votes
1
reply
177
views
How to save the AlphaFold PAE plot as an image in ChimeraX using command line ?
PAE
ChimeraX
AlphaFold
updated 14 hours ago by
LauferVA
4.7k • written 2 days ago by
Picasa
▴ 680
1
vote
0
replies
62
views
News:
Building an mmCIF parser for enzyme structure analysis — feedback & ideas welcome!
mmcif
updated 15 hours ago by
GenoMax
150k • written 17 hours ago by
Shravya
• 0
1
vote
0
replies
70
views
News:
LAST CALL - Bioinformatics Pipeline Development with Nextflow - May 19-21, 2025 (online) - Apply Now
nextflow
rnaseq
workshop
17 hours ago by
ecSeq Bioinformatics
▴ 20
1
vote
2
replies
180
views
RNA-seq, three comparisons - stick to one linear model, or use separate models?
statistics
mixed_effect_model
linear_model
stats
RNAseq
10 hours ago by
noodlejackson
▴ 40
0
votes
1
reply
105
views
Using Principal Components may muddle the detecting power of a GWAS?
q-value
covariates
genomic-inflation
pca
gwas
updated 18 hours ago by
DBScan
▴ 470 • written 20 hours ago by
AndrMod
• 0
0
votes
0
replies
81
views
Use Seed or Full Alignments from Pfam HMM as a filter step
hmm
sequences
metagenomic
20 hours ago by
Janelyn123
• 0
1
vote
4
replies
230
views
Helicobacter pylori low-rate alignment
bioinformatics
transcriptomics
RNA-seq
updated 11 hours ago by
GenoMax
150k • written 1 day ago by
Ariadna
▴ 20
14
votes
3
replies
11k
views
Can The Wget Command Be Used To Download Pdbs
pdb
updated 22 hours ago by
Calvin
• 0 • written 13.5 years ago by
Harpalss
▴ 310
0
votes
3
replies
260
views
Is a log2 transformation an essential step in preparing expression data for machine learning?
Machinelearning
microarray
preproccessing
updated 1 day ago by
Mensur Dlakic
★ 29k • written 1 day ago by
Sib
▴ 60
0
votes
2
replies
853
views
How to re-annotate the structure of gene against a known genomic file
RNA-Seq
updated 1 day ago by
Mensur Dlakic
★ 29k • written 5.9 years ago by
Kanoo
• 0
0
votes
1
reply
185
views
ONT Live-Basecalling Issue?
ONT
minKNOW
live-basecalling
updated 1 day ago by
GenoMax
150k • written 2 days ago by
Akshay
• 0
3
votes
4
replies
266
views
Identify gene from sequence at scale in non-model organisms
name
sequence
gene
updated 1 day ago by
shelkmike
★ 1.5k • written 2 days ago by
matthewmerkin32
• 0
0
votes
0
replies
130
views
News:
Cellosaurus release 52 is available
database
line
cell
knowledgebase
biocuration
2 days ago by
Amos Bairoch
▴ 130
0
votes
2
replies
252
views
WGCNA
problem
updated 2 days ago by
GenoMax
150k • written 3 days ago by
香
• 0
0
votes
0
replies
241
views
Seeking Advice on Causal Inference for Treatment Effect Prediction (Small Sample, Genomic Covariates)
inference
causal
2 days ago by
oghzzang
▴ 50
5
votes
5
replies
432
views
Batch effect
RNAseq
Batch-Effect
1 day ago by
Fatemeh
▴ 40
0
votes
4
replies
367
views
convert Gene ID to Gene Symbol
gene-id
updated 2 days ago by
pinheirofabiano
▴ 110 • written 5 days ago by
majorprogeria
• 0
0
votes
1
reply
194
views
News:
Structural bioinformatics workshop
Structural
bioinformatics
updated 3 days ago by
GenoMax
150k • written 3 days ago by
reetesh
• 0
1
vote
10
replies
548
views
How to test the similarity of two gene expression profiles
test
gene
sets
updated 3 days ago by
JustinZhang
▴ 130 • written 4 days ago by
htchd
• 0
3
votes
3
replies
230
views
Which are the different kinds of GeneID that exist?
R
RNA-Seq
3 days ago by
pinheirofabiano
▴ 110
0
votes
0
replies
144
views
Error using DWLS
deconvolution
DWLS
RNAseq
3 days ago by
Ana
• 0
0
votes
5
replies
1.7k
views
How to remove this module selection error?
WGCNA
updated 3 days ago by
香
• 0 • written 2.3 years ago by
gupta00aritra
• 0
0
votes
1
reply
870
views
bbsplit : How to configure ambiguous/ambiguous2 parameters in order to consider as unmapped reads, reads mapped on both human/mouse references.
bbsplit
nextflow
updated 3 days ago by
GenoMax
150k • written 3 days ago by
ZheFrench
▴ 590
1
vote
2
replies
219
views
Limma for DEG aalysis
R
DEG
limma
ANALYSIS
3 days ago by
rahma.khoualdia
• 0
0
votes
0
replies
182
views
deletion impact
vcf
updated 3 days ago by
GenoMax
150k • written 3 days ago by
HarperReed
• 0
0
votes
2
replies
236
views
Help finding GTEx v8 file
GTEx
SHAPEIT2
2 days ago by
蒲蒲
• 0
2
votes
5
replies
287
views
Idat file processing issue with command line cli
CanineHTS
IDAT
3 days ago by
1769mkc
★ 1.2k
0
votes
0
replies
151
views
is there a way to get --ref paths to prioritize my order with vg call?
call
vg
vgcall
2 hours ago by
PolenP
• 0
0
votes
1
reply
211
views
Has anyone tried used simple ML models to identify virulence genes?
genetics
R
classifier
genomics
machinelearning
updated 3 days ago by
Mensur Dlakic
★ 29k • written 4 days ago by
Jack
• 0
0
votes
4
replies
328
views
What does vg autoindex mpmap do?
vg
mpmap
autoindex
13 hours ago by
Ziyue
• 0
5
votes
4
replies
373
views
How to keep the same view for different AlphaFold 3 simulations in ChimeraX?
chimerax
alphafold
3
updated 3 days ago by
LauferVA
4.7k • written 4 days ago by
Picasa
▴ 680
0
votes
0
replies
171
views
filtering genes before or after the correlation?
co-expression
feature
selection
correlation
4 days ago by
tyasird
▴ 10
0
votes
16
replies
735
views
Which tools use or rely on the XA tag in SAM/BAM/CRAM files?
cram
XA
compress
updated 3 days ago by
JustinZhang
▴ 130 • written 5 days ago by
Maarten
• 0
2
votes
4
replies
291
views
Which additional files are needed for the -taxids option in blast?
decontamination
taxids
blastn
updated 4 days ago by
GenoMax
150k • written 4 days ago by
WaspInSpace
• 0
0
votes
0
replies
187
views
Job:
Assistant, Associate, or Full Project Scientist in Neurobiology
Biology
Neuroscience
Neurobiology
4 days ago by
UCSDBioSci
▴ 30
1
vote
3
replies
303
views
Is Centering and Scaling Essential for Expression Data as Input for Machine Learning Algorithms?
featureselection
microarray
preprocessing
1 day ago by
Sib
▴ 60
0
votes
0
replies
195
views
News:
Online Course: Machine learning for multi-omics integration (9–11 June)
Machine-Learning
Mult-Omics
4 days ago by
Physalia-courses
★ 2.6k
0
votes
2
replies
275
views
Single Nuclei RNA-Sequencing Analysis Without CellRanger
CellRanger
single-cell
snRNA-seq
3 days ago by
16_masques.vole
• 0
120,980 results • Page
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Recent Votes
How to keep the same view for different AlphaFold 3 simulations in ChimeraX?
Answer: How to keep the same view for different AlphaFold 3 simulations in ChimeraX?
Answer: Chip-seq Analysis
Comment: ChIP-seq gene blacklist for Rattus norvegicus
C: Filtering a multi-person structural variant VCF file by type of SV
Comment: Helicobacter pylori low-rate alignment
STAR aligner - Is this still maintained?
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Recent Replies
Answer: Using DEXseq on kallisto tsv outputs ?
by
Gordon Smyth
★ 7.9k
Inputting transcript (RNA isoform) quantifications from kallisto into tools like DEXSeq that are designed for exons is not ideal because th…
Comment: ChIP-seq gene blacklist for Rattus norvegicus
by
GenoMax
150k
https://kilthub.cmu.edu/articles/dataset/Alternate_gene_annotations_for_rat_macaque_and_marmoset_for_single_cell_RNA_and_ATAC_analyses/2117…
Comment: ChIP-seq gene blacklist for Rattus norvegicus
by
RD
▴ 20
Were you able to find the list of regions? Thank you!
Comment: Extracting Variant Sequences from 1000 Genomes VCF File and Mapping to Canonical
by
cmdcolin
★ 4.2k
doesn't solve your problem exactly but I stumbled on this blog post awhile back https://kalis.louisaslett.com/articles/lct_example.html#re…
Answer: Extract length of sample sequence from vcf
by
cmdcolin
★ 4.2k
Not sure if it helps but I saw this impressive one-liner in a blog post that 'reconstructs the sequence for the 1000 genomes samples' http…
Comment: RNA-seq, three comparisons - stick to one linear model, or use separate models?
by
noodlejackson
▴ 40
Thank you @ATpoint
Comment: Is it reasonable to do "Gene Regulatory Network Inference" for each cell type an
by
Hao
▴ 10
it's a bioarxiv so I didn't provide the link. Anyway, link added and I changed the language.
Comment: Is it reasonable to do "Gene Regulatory Network Inference" for each cell type an
by
GenoMax
150k
Please edit the answer above and provide a link (either PubMed or direct journal) for the paper. Just saying you did "X" is not providing a…
Comment: Helicobacter pylori low-rate alignment
by
GenoMax
150k
> What is the best trade off in this scenario? As noted earlier, this data is ~ 15 yr old at this point i.e. from the dawn of NGS tech. It…
Answer: Is it reasonable to do "Gene Regulatory Network Inference" for each cell type an
by
Hao
▴ 10
Sure. In [one of our preprints][1], we did sort of a similar thing on cells from different time points. [1]: https://www.biorxiv.org/c…
Answer: Blastx issue
by
colindaven
7.4k
You could also use an ORF finder like transdecoder or prodigal as an additional check. Many lncRNAs might have very short ORFs, but are unl…
Answer: TPM vs FPKM divergence at high values
by
Istvan Albert
102k
Both TPM and FPKM will normalize to the so-called "library size", which is how many mapped reads are in each sample. So, that is not what c…
Comment: Extract length of sample sequence from vcf
by
Istvan Albert
102k
make sure the variants are normalized and properly decomposed, also maybe show that VCF row as well, This example you gave seems to show…
Comment: Helicobacter pylori low-rate alignment
by
GenoMax
150k
> using bowtie 2 and the genomic annotation taken from https://www.ncbi.nlm.nih.gov/datasets/genome/GCF_000008525.1/ Did you see folowing …
Answer: What is the best way to know if a pair of genes is random?
by
shelkmike
★ 1.5k
I don't know about the best way, but one method to do this, I think, could look as follows:<br> 1) Take all possible pairs of genes and fo…
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