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120,972 results • Page
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0
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14
views
RNA-seq, three comparisons - stick to one linear model, or use separate models?
statistics
mixed_effect_model
linear_model
stats
RNAseq
1 hour ago by
noodlejackson
▴ 40
0
votes
3
replies
143
views
Is a log2 transformation an essential step in preparing expression data for machine learning?
Machinelearning
microarray
preproccessing
updated 2 hours ago by
Mensur Dlakic
★ 29k • written 13 hours ago by
Sib
▴ 60
0
votes
2
replies
770
views
How to re-annotate the structure of gene against a known genomic file
RNA-Seq
updated 2 hours ago by
Mensur Dlakic
★ 29k • written 5.9 years ago by
Kanoo
• 0
836
votes
167
replies
173k
views
111 follow
News:
The Biostar Handbook. A bioinformatics e-book for beginners.
training
handbook
updated 13 hours ago by
Biostar
3.4k • written 8.4 years ago by
Istvan Albert
102k
0
votes
0
replies
43
views
Helicobacter pylori low-rate alignment
bioinformatics
transcriptomics
RNA-seq
6 hours ago by
Ariadna
▴ 20
0
votes
1
reply
103
views
ONT Live-Basecalling Issue?
ONT
minKNOW
live-basecalling
updated 1 hour ago by
GenoMax
150k • written 1 day ago by
Akshay
• 0
0
votes
1
reply
116
views
Identify gene from sequence at scale in non-model organisms
name
sequence
gene
updated 5 hours ago by
shelkmike
★ 1.5k • written 1 day ago by
matthewmerkin32
• 0
0
votes
0
replies
81
views
News:
Cellosaurus release 52 is available
database
line
cell
knowledgebase
biocuration
1 day ago by
Amos Bairoch
▴ 130
0
votes
2
replies
197
views
WGCNA
problem
updated 23 hours ago by
GenoMax
150k • written 2 days ago by
香
• 0
0
votes
0
replies
162
views
Seeking Advice on Causal Inference for Treatment Effect Prediction (Small Sample, Genomic Covariates)
inference
causal
1 day ago by
oghzzang
▴ 50
0
votes
0
replies
92
views
How to save the AlphaFold PAE plot as an image in ChimeraX using command line ?
PAE
ChimeraX
AlphaFold
1 day ago by
Picasa
▴ 670
5
votes
5
replies
358
views
Batch effect
RNAseq
Batch-Effect
8 hours ago by
Fatemeh
▴ 40
0
votes
4
replies
312
views
convert Gene ID to Gene Symbol
gene-id
updated 1 day ago by
pinheirofabiano
▴ 110 • written 4 days ago by
majorprogeria
• 0
0
votes
4
replies
218
views
TPM vs FPKM divergence at high values
tpm
fpkm
normalisation
updated 8 hours ago by
ATpoint
87k • written 1 day ago by
megancgrdnr
• 0
0
votes
1
reply
164
views
Blastx issue
Long
coding
non
blastx
RNA
updated 1 day ago by
JustinZhang
▴ 130 • written 2 days ago by
Avik
• 0
0
votes
1
reply
146
views
News:
Structural bioinformatics workshop
Structural
bioinformatics
updated 2 days ago by
GenoMax
150k • written 2 days ago by
reetesh
• 0
1
vote
10
replies
480
views
How to test the similarity of two gene expression profiles
test
gene
sets
updated 1 day ago by
JustinZhang
▴ 130 • written 2 days ago by
htchd
• 0
3
votes
3
replies
185
views
Which are the different kinds of GeneID that exist?
R
RNA-Seq
2 days ago by
pinheirofabiano
▴ 110
0
votes
0
replies
106
views
Error using DWLS
deconvolution
DWLS
RNAseq
2 days ago by
Ana
• 0
0
votes
5
replies
1.7k
views
How to remove this module selection error?
WGCNA
updated 2 days ago by
香
• 0 • written 2.3 years ago by
gupta00aritra
• 0
0
votes
1
reply
622
views
bbsplit : How to configure ambiguous/ambiguous2 parameters in order to consider as unmapped reads, reads mapped on both human/mouse references.
bbsplit
nextflow
updated 2 days ago by
GenoMax
150k • written 2 days ago by
ZheFrench
▴ 590
1
vote
2
replies
168
views
Limma for DEG aalysis
R
DEG
limma
ANALYSIS
2 days ago by
rahma.khoualdia
• 0
0
votes
0
replies
133
views
deletion impact
vcf
updated 2 days ago by
GenoMax
150k • written 2 days ago by
HarperReed
• 0
0
votes
2
replies
183
views
Help finding GTEx v8 file
GTEx
SHAPEIT2
1 day ago by
蒲蒲
• 0
2
votes
5
replies
239
views
Idat file processing issue with command line cli
CanineHTS
IDAT
2 days ago by
1769mkc
★ 1.2k
0
votes
0
replies
114
views
What is the best way to know if a pair of genes is random?
terms
genes
go
2 days ago by
Ana
• 0
0
votes
0
replies
109
views
is there a way to get --ref paths to prioritize my order with vg call?
call
vgcall
2 days ago by
PolenP
• 0
0
votes
1
reply
170
views
Has anyone tried used simple ML models to identify virulence genes?
genetics
R
classifier
genomics
machinelearning
updated 2 days ago by
Mensur Dlakic
★ 29k • written 2 days ago by
Jack
• 0
0
votes
3
replies
237
views
What does vg autoindex mpmap do?
vg
mpmap
autoindex
updated 22 hours ago by
Jordan M Eizenga
▴ 690 • written 2 days ago by
Ziyue
• 0
3
votes
4
replies
312
views
How to keep the same view for different AlphaFold 3 simulations in ChimeraX?
chimerax
alphafold
3
updated 1 day ago by
LauferVA
4.7k • written 3 days ago by
Picasa
▴ 670
0
votes
0
replies
129
views
filtering genes before or after the correlation?
co-expression
feature
selection
correlation
3 days ago by
tyasird
▴ 10
0
votes
16
replies
656
views
Which tools use or rely on the XA tag in SAM/BAM/CRAM files?
cram
XA
compress
updated 2 days ago by
JustinZhang
▴ 130 • written 4 days ago by
Maarten
• 0
2
votes
4
replies
240
views
Which additional files are needed for the -taxids option in blast?
decontamination
taxids
blastn
updated 3 days ago by
GenoMax
150k • written 3 days ago by
WaspInSpace
• 0
0
votes
0
replies
146
views
Job:
Assistant, Associate, or Full Project Scientist in Neurobiology
Biology
Neuroscience
Neurobiology
3 days ago by
UCSDBioSci
▴ 30
1
vote
3
replies
253
views
Is Centering and Scaling Essential for Expression Data as Input for Machine Learning Algorithms?
featureselection
microarray
preprocessing
13 hours ago by
Sib
▴ 60
0
votes
0
replies
157
views
News:
Online Course: Machine learning for multi-omics integration (9–11 June)
Machine-Learning
Mult-Omics
3 days ago by
Physalia-courses
★ 2.6k
0
votes
2
replies
233
views
Single Nuclei RNA-Sequencing Analysis Without CellRanger
CellRanger
single-cell
snRNA-seq
2 days ago by
16_masques.vole
• 0
0
votes
0
replies
161
views
Demultiplexing samples when barcodes repeat: Any advice?
seurat
demultiplex
singlecell
scRNAseq
3 days ago by
tia
• 0
1
vote
1
reply
264
views
Unable to generate hierarchical and circle plot using CellChat
cellchat
scRNAseq
Seurat
4 days ago by
S
• 0
0
votes
8
replies
524
views
Discrepancy between positions on IGV and NCBI's PrimerBLAST
igv
primer
blast
2 days ago by
Rozita
▴ 40
0
votes
2
replies
238
views
Steps for meta-analysis
Meta-Analysis
differential-expression
updated 3 days ago by
Ram
45k • written 4 days ago by
majorprogeria
• 0
5
votes
1
reply
258
views
News:
The IGB team needs your help! Write a letter of support to keep the Integrated Genome Browser running!
IGB
Integrated-Genome-Browser
Funding
updated 4 days ago by
microfuge
★ 2.0k • written 4 days ago by
Nowlan Freese
▴ 900
0
votes
0
replies
192
views
how pd tag were defined in gaf file
vg
4 days ago by
chuanj8848
• 0
4
votes
2
replies
299
views
SRA Toolkit - three fastq files - which is which?
scrna
sratoolkit
updated 4 days ago by
GenoMax
150k • written 4 days ago by
Neat
• 0
0
votes
0
replies
158
views
Using DEXseq on kallisto tsv outputs ?
alignement
DEXseq
kallisto
4 days ago by
Gerard
▴ 10
1
vote
0
replies
191
views
Herald:
The Biostar Herald for Wednesday, April 09, 2025
herald
4 days ago by
Biostar
3.4k
6
votes
6
replies
472
views
Methods for directional pathway analysis of DEGs?
Pathway-analysis
ORA
GSEA
Limma
updated 3 days ago by
Gordon Smyth
★ 7.9k • written 6 days ago by
Steven
▴ 10
0
votes
0
replies
170
views
GTDB-tk classify_wf
GTDB-tk
classification
taxonomic
4 days ago by
pranjalarun10
• 0
0
votes
1
reply
535
views
Help finding proteases that most likely cleaved a peptide from a full-length protein
R
peptides
proteasix
updated 4 days ago by
Fatemeh
▴ 40 • written 18 months ago by
georgiamorgannn
• 0
0
votes
0
replies
161
views
protease candidates for my protein of interest
cleaving-site
proteases
protein
4 days ago by
Fatemeh
▴ 40
120,972 results • Page
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A: Annotatepeaks Function From Homer
Answer: Batch effect
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Comment: ONT Live-Basecalling Issue?
by
GenoMax
150k
Did this block eliminate itself over time? It looks like ~4 M initial reads in your run are in "paused" state. Have not heard of this happe…
Answer: Is a log2 transformation an essential step in preparing expression data for mach
by
Mensur Dlakic
★ 29k
> Is a log2 transformation an essential step in preparing the expression data for machine learning, or can the normalized raw expression da…
Comment: How to re-annotate the structure of gene against a known genomic file
by
Mensur Dlakic
★ 29k
If you look above from this writing, this question was asked 5.9 years ago. If you click on user's name, you will see that they last visite…
Comment: Is a log2 transformation an essential step in preparing expression data for mach
by
Sib
▴ 60
I would greatly appreciate it if you could refer me to a tutorial on conducting feature selection for microarray data using some (or all) o…
Comment: Identify gene from sequence at scale in non-model organisms
by
shelkmike
★ 1.5k
I don't understand why you think that you need some gene names to perform GO annotation. Just upload your proteins to PANNZER. It is an onl…
Comment: How to re-annotate the structure of gene against a known genomic file
by
Olayemi F.
• 0
Hey Kanoo, How did this later turn out for you?
Comment: Batch effect
by
Fatemeh
▴ 40
Thanks very much.
Comment: Is a log2 transformation an essential step in preparing expression data for mach
by
ATpoint
87k
You're shooting buzz words as in the question before. Please read the underlying literature and follow guided tutorials. There is no genera…
Comment: TPM vs FPKM divergence at high values
by
ATpoint
87k
Please show the functions to calculate this. Both transformations are simple division and multiplication operations with the column sums of…
Comment: Is Centering and Scaling Essential for Expression Data as Input for Machine Lear
by
Sib
▴ 60
Sorry, is log2 taransformation also needed? Or we can apply ML methods on expression data without it?
Comment: TPM vs FPKM divergence at high values
by
pinheirofabiano
▴ 110
I don't have a better mathematical explanation, but FPKMs are not reliable for gene expression studies, there are many discussions about t…
Comment: TPM vs FPKM divergence at high values
by
megancgrdnr
• 0
Thank you for your answer! Do you have any sense of why the expression values diverge at higher-expressed genes? I can't see anything in th…
Comment: What does vg autoindex mpmap do?
by
Jordan M Eizenga
▴ 690
GCSA indexing certainly can be time consuming. On a reasonably well-behaved graph (such as those derived from VCFs, or usually those from t…
Comment: WGCNA
by
1769mkc
★ 1.2k
what your gene expression matrix size or dimension?
Answer: WGCNA
by
PFSXR
• 0
There are too few valid genes in the input matrix expr_filtered, possibly because after the goodSamplesGenes filtering, only the grey modul…
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