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Recent Replies
Comment: Filter snRNA-seq .fastq files based on barcodes
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GenoMax
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Can you post an exact example of what your fastq headers look like?
`names` file needs to have only one entry per line without columns.
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From the [--chr-set documentation][1]:
"--chr-set changes the chromosome set. The first parameter specifies the number of diploid autoso…
Comment: Good QC Package (RSeQC or alternative) for Salmon mapping/quantification results
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squidqid
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MultiQC is very helpful. From my understanding, however, MultiQC will not parse the information from useful auxiliary outputs like .../aux_…
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It is generally expected in communities like this that you read the manual first which covers this https://bioconductor.org/packages/releas…
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If you are using single cell data then you really should be using `alevin-fry/simpleaf` packages ([**LINK**][1]) instead of `salmon`. There…
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can you show us that line in the VCF:
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Since the host is always going to be there you can do the mapping to a combined three transcriptome pool, as a test/first-pass. That will g…
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squidqid
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Did you ever get an answer to this question?
Comment: Lacking exons in gtf file of a virus' genome
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ZuelTech
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I mean, can I map all lists of the libraries ( HAZV-infected, LGTV-infected, uninfected) to the HAZV genome? Can I also map them to the LGT…
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> Then, I want to map the libraries to each of the virus genome. Is this possible?
Possible but will that be logical. If you have all thr…
Comment: Lacking exons in gtf file of a virus' genome
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Thank you! Btw, going back to mapping, I’m working with a vector infected with HAZV and LGTV virus. I have libraries with the host infected…
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Yes, that you can do as well. Then you don't need to use STAR. But I would add the host transcriptome as a decoy there.
Comment: Lacking exons in gtf file of a virus' genome
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ZuelTech
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I see, thank you so much! I’ll try this method. Can I also use Salmon in quantification?
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> Does it mean I don’t need to put a gtf file when I do mapping to HAZV
> genome?
Indeed, unless you want to generate the count file in ST…
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