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120,771 results • Page
1 of 2416
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How to perform burden test using plink /seq
Burden
Test
11 minutes ago by
aiswaryabioinfo
▴ 30
0
votes
3
replies
31
views
Higher number of reads mapped to transcriptome vs genome in star mapping
riboseq
star
bam
transcriptome
alignment
updated 14 minutes ago by
i.sudbery
21k • written 1 hour ago by
paguirreazorin
• 0
0
votes
0
replies
31
views
Visualsation_of_reads depth
read_depth_measure
variant
calling
coverage
updated 2 hours ago by
Nicolas Rosewick
11k • written 2 hours ago by
AIMAR
• 0
7
votes
5
replies
582
views
Tool:
AliNe (Alignment in Nextflow)
nextflow
alignment
2 hours ago by
Juke34
9.2k
0
votes
0
replies
32
views
construct PKN
REGULATORY
PKN
MOUSE
NETWORKS
GENE
2 hours ago by
rahma.khoualdia
• 0
835
votes
169
replies
170k
views
110 follow
News:
The Biostar Handbook. A bioinformatics e-book for beginners.
training
handbook
updated 6 months ago by
Biostar
3.4k • written 8.3 years ago by
Istvan Albert
102k
0
votes
2
replies
83
views
Tool for automatic immune cell annotation in MOUSE?
immune
single-cell
annotation
updated 35 minutes ago by
theHumanBorch
▴ 240 • written 5 hours ago by
txema.heredia
▴ 210
4
votes
12
replies
6.9k
views
12 follow
How to draw venn diagram for two CNV lists?
venn
updated 5 hours ago by
5heikki
11k • written 10.3 years ago by
lyz10302012
▴ 480
0
votes
1
reply
64
views
merging diploid and tetraploid vcfs
bcftools
diploid
tetraploid
updated 5 hours ago by
colindaven
7.3k • written 6 hours ago by
analyst
▴ 60
0
votes
0
replies
46
views
TMM normalized CPM
TPM
CPM
TMM
7 hours ago by
maryak
▴ 20
2
votes
3
replies
134
views
Downloading full list of Homo sapiens genes
Genes
updated 8 hours ago by
GenoMax
150k • written 13 hours ago by
nigussie.amu
• 0
0
votes
0
replies
45
views
Normlizatiom in DiffBind
DiffBind
CHIP-Seq
9 hours ago by
Siqi
• 0
0
votes
1
reply
72
views
Obtain genome coordinates for a DNA sequence using ENSEMBL API
Ensembl
API
updated 11 hours ago by
GenoMax
150k • written 13 hours ago by
Ishan
• 0
0
votes
0
replies
54
views
Forum:
Decoding Corvid Intelligence: Genomics, AI & the Genetics of Cognition
neurogenetics
comparative-genomics
bioinformatics
genomics
machine-learning
updated 12 hours ago by
Ram
45k • written 13 hours ago by
EvoGenExplorer
• 0
2
votes
3
replies
160
views
GBWT Construction Error: "Sources X and Y both have node Z" When Building GBZ from GFA
vg
updated 11 hours ago by
Jouni Sirén
▴ 580 • written 19 hours ago by
Sauers
▴ 10
8
votes
16
replies
391
views
6 follow
prokka with too many "hypothetical proteins" and genome comparison
comparison
genome
galaxy
prokka
2 hours ago by
avinci1
▴ 20
0
votes
1
reply
124
views
Split paired ended hWGS FASTq files to simulate difference sequence coverage
whole-genome-sequencing
wgs
FASTQ
coverage
updated 19 hours ago by
shelkmike
★ 1.5k • written 1 day ago by
DJBill
▴ 20
0
votes
1
reply
142
views
OMA use case
oma
omastandalone
updated 20 hours ago by
Adrian Altenhoff
★ 1.1k • written 1 day ago by
nmalexan
• 0
0
votes
0
replies
67
views
News:
Machine Learning for Longitudinal Data with Python – Online Course (6-9 May)
MachineLearning
LongitudinalData
DeepLearning
Omics
20 hours ago by
Physalia-courses
★ 2.6k
1
vote
2
replies
138
views
Recommended workflow to pre-process and demultiplex scRNAseq data based on the HTOs and ADTs
ADT
HTO
demultiplexing
scRNAseq
5 hours ago by
mete.han.celebi
• 0
0
votes
3
replies
129
views
bwa-mem keeps failing to locate the index files
bwa
bwa-mem
updated 22 hours ago by
ATpoint
87k • written 22 hours ago by
pairedttest
▴ 30
1
vote
5
replies
309
views
6 follow
Efficient Gene Prediction on Large Eukaryotic Genomes
tools
prediction
gene
updated 21 hours ago by
Joe
22k • written 3 days ago by
Shuo
• 0
0
votes
0
replies
62
views
Olink - OlinkAnalyze Package - olink_umap_plot function
Olink
proteomics
22 hours ago by
kristina
• 0
3
votes
0
replies
91
views
Herald:
The Biostar Herald for Wednesday, March 19, 2025
herald
1 day ago by
Biostar
3.4k
0
votes
0
replies
73
views
Cosmic database update issue in annovar
annovar
cosmic
genomics
updated 23 hours ago by
Ram
45k • written 1 day ago by
abhijitswain
• 0
1
vote
1
reply
245
views
Transforming diploid GT on male X and Y chromosome to haploid
VCF
genotype
gVCF
1 day ago by
Michaela
▴ 10
3
votes
2
replies
195
views
How do I figure out pangenome location of hg38 coordinates?
pangenome
hg38
vg
1 day ago by
a5864557
▴ 10
3
votes
6
replies
272
views
How I can curate all exon start-end positions for 50 genes (to create GTF file)?
gtf
curation
gene
2 hours ago by
Esra
• 0
0
votes
0
replies
91
views
generating manhattan plot through hail
plot
manhattan
hail
visualizations
gwas
1 day ago by
nitya
• 0
0
votes
0
replies
93
views
Hard Filtering Differences between WES and WGS
WES
WGS
Hard-Filtering
updated 23 hours ago by
Ram
45k • written 1 day ago by
SIMONE
• 0
0
votes
3
replies
197
views
Am I accounting for replicates correctly in pseudobulk analysis?
deseq2
single-cell
pseudobulk
updated 1 day ago by
ATpoint
87k • written 1 day ago by
Aspire
▴ 370
2
votes
4
replies
334
views
Is there too little variation to detect meaningful gene changes?
DESEQ
updated 1 day ago by
Istvan Albert
102k • written 3 days ago by
Megan
▴ 50
2
votes
4
replies
231
views
nanopore SNV callers returns empty VCF
snv_caller
nanopore
9 hours ago by
Meghan.T
• 0
15
votes
3
replies
1.1k
views
Tutorial:
Avoiding the Pitfalls of the Anaconda License: A Practical Guide
conda
Anaconda
license
19 hours ago by
Juke34
9.2k
0
votes
1
reply
131
views
How many cells are needed in subcluster to draw conclusions?
immunology
T-cells
scRNA-seq
updated 1 day ago by
ATpoint
87k • written 1 day ago by
S
• 0
0
votes
1
reply
133
views
Question about assigning gene names, tximport and DESeq2
tximport
deseq2
updated 1 day ago by
Istvan Albert
102k • written 1 day ago by
bioinfo
▴ 150
2
votes
3
replies
225
views
RNASeq: Confused about adapters with barcodes and seq company's filtering
atria
rnaseq
adapter-trimming
updated 10 hours ago by
GenoMax
150k • written 2 days ago by
Davor
• 0
1
vote
2
replies
197
views
VEP won't show symbols for all variants; SnpEff will, but won't for others
vep
snpeff
updated 1 day ago by
Jeremy Leipzig
23k • written 2 days ago by
mary.v.volkova
▴ 20
0
votes
2
replies
145
views
How to plot LMER estimate values in heatmap ?
cell
scRNAseq
TF
lmer
transcription
22 hours ago by
carolofharvest
▴ 50
1
vote
2
replies
847
views
News:
Final Call - Next-Generation Sequencing Data Analysis: A Practical Introduction (April 2-4 in Munich) - Only 2 seats left
Workshop
RNAseq
Variant-Calling
Illumina
DNAseq
2 days ago by
ecSeq Bioinformatics
▴ 10
0
votes
1
reply
141
views
Guidance Needed: Best Practices for Handling Technical Replicates in RNA-seq Analysis
tehnical-replicates
DESeq2
RNA-Seq
updated 1 day ago by
Ram
45k • written 2 days ago by
hellothere
• 0
0
votes
2
replies
163
views
Can anyone help me download the dbSNP data for Bos taurus ARS-UCD1.3?
bioinformatics
GWAS
genetics
WGS
8 hours ago by
K
• 0
0
votes
1
reply
178
views
Co-expression analysis of miRNAs and target mRNAs
miRNA
targets
co-expression
miRNA-mRNA
updated 2 days ago by
i.sudbery
21k • written 2 days ago by
baibhu1234
▴ 40
0
votes
0
replies
129
views
Beagle Imputation
Beagle
Imputation
2 days ago by
mahlet.teka
• 0
0
votes
0
replies
250
views
Issue with bam-readcount and fpfilter.pl: Saying "failed to get readcounts for variant allele"
bam-readount
somaticsniper
updated 2 days ago by
Pierre Lindenbaum
165k • written 2 days ago by
1phamcha2
• 0
2
votes
2
replies
224
views
miRNA-seq: QC reports and workflow
miRNA-seq
ION-Torrent
RNA-seq
FASTQC
1 day ago by
omicon
▴ 40
1
vote
0
replies
124
views
How to Run Large-Scale Foldseek Comparisons (Human vs. C. elegans)
c.elegans
Foldseek
Genes
Orthologs
Homologs
2 days ago by
bioinformatics_rk
▴ 10
0
votes
1
reply
210
views
Recommendations for Reference-guided de novo assembly assembly approaches or pipelines?
ont
assembly
wgs
nanopore
genomics
updated 2 days ago by
shelkmike
★ 1.5k • written 4 days ago by
Mark
▴ 30
0
votes
1
reply
186
views
Per Base Sequence Content FastQC
RNA-seq
quality-control
updated 2 days ago by
shelkmike
★ 1.5k • written 3 days ago by
Ariadna
▴ 20
3
votes
2
replies
255
views
Seeking Advice on Handling Multiple Datasets for Differential Analysis in Transcriptomics
Data-Integration
Differential-Analysis
Transcriptomics
Data-Normalization
2 days ago by
Riley J
• 0
120,771 results • Page
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Answer: Downloading full list of Homo sapiens genes
Answer: Downloading full list of Homo sapiens genes
How To Ask Good Questions On Technical And Scientific Forums
Answer: Avoiding the Pitfalls of the Anaconda License: A Practical Guide
The Biostar Herald for Wednesday, March 19, 2025
Comment: prokka with too many "hypothetical proteins" and genome comparison
Answer: Pre-Alignment process
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Recent Replies
Answer: Higher number of reads mapped to transcriptome vs genome in star mapping
by
i.sudbery
21k
I can think of a possibility Consider: You aligned reads to transcriptome, that contains two isoforms for a gene. Reads that come fro…
Comment: Higher number of reads mapped to transcriptome vs genome in star mapping
by
i.sudbery
21k
You might also try seeing how many alignments to each before deduplication. And also looking at how many *reads" there are, rather than how…
Comment: Higher number of reads mapped to transcriptome vs genome in star mapping
by
i.sudbery
21k
Can you clarify what you mean by "isoform selection"?
Comment: Tool for automatic immune cell annotation in MOUSE?
by
theHumanBorch
▴ 240
When annotating, I like to combine canonical markers with automated annotation systems - the go to for mouse immune populations I use is 1…
Answer: AliNe (Alignment in Nextflow)
by
Juke34
9.2k
`Last`is now present in AliNe v1.3.0 Before that `bwa-mem2` and `salmon` have been added in AliNe v1.2.0
Comment: prokka with too many "hypothetical proteins" and genome comparison
by
avinci1
▴ 20
Thank you! I will try that!
Comment: Pre-Alignment process
by
AIMAR
• 0
thanks for the sharing.
Comment: prokka with too many "hypothetical proteins" and genome comparison
by
avinci1
▴ 20
There are 64 contigs for one genome and 114 for the other one. The sequencing service should be reputable. I obtained the same output with …
Comment: How I can curate all exon start-end positions for 50 genes (to create GTF file)?
by
Esra
• 0
Thank you for your answee.I also looked these AGAT tools you suggested, but I wasn't sure how to use it (I am actually a medical doctor and…
Comment: How I can curate all exon start-end positions for 50 genes (to create GTF file)?
by
Esra
• 0
Thank you for your reply and for the clarification. Yes, I wanted to curate the MANE Select (Canonical) transcript's exons, so thanks for t…
Comment: Tool for automatic immune cell annotation in MOUSE?
by
Bastien Hervé
6.1k
Look at the gene markers driving each cluster to see if you can identify specific cell population. You can also fetch from online databases…
Comment: Identifying primer sequences from raw FASTQ files
by
michael.ante
★ 4.0k
The primers don't need to be unambiguous sequences. Often to improve binding, they are given with ambiguous DNA-letters, which in the end m…
Comment: merging diploid and tetraploid vcfs
by
colindaven
7.3k
How are you calling tetraploid variants out of interest ?
Answer: How to draw venn diagram for two CNV lists?
by
5heikki
11k
[This][1] is my favorite online tool for list comparisons [1]: https://molbiotools.com/listcompare.php
Comment: Recommended workflow to pre-process and demultiplex scRNAseq data based on the H
by
mete.han.celebi
• 0
Thank you very much! Yes I haven't seen this. That is very valuable!
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