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120,856 results • Page
1 of 2418
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Votes
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0
votes
0
replies
15
views
Count matrices plotting
count
matrix
scanpy
18 minutes ago by
NIkita
• 0
0
votes
0
replies
8
views
Why is the IC50 of the low-risk group always lower than that of the high-risk group?
OncoPredict
46 minutes ago by
nuorain
▴ 40
0
votes
13
replies
258
views
Lacking exons in gtf file of a virus' genome
gtf
exon
Mapping
Hazara
VirusGenome
updated 3 hours ago by
GenoMax
150k • written 9 hours ago by
ZuelTech
• 0
0
votes
0
replies
38
views
News:
Introduction to Epigenomics course
Epigenomics
RNA-seq
ATAC-seq
Chip-seq
HI-C
6 hours ago by
Physalia-courses
★ 2.6k
0
votes
1
reply
53
views
Remove batch effect RnaSeq (RUVg)
ruvg
batch
rnaseq
remove
updated 1 hour ago by
ATpoint
87k • written 6 hours ago by
aLex97
• 0
835
votes
169
replies
172k
views
110 follow
News:
The Biostar Handbook. A bioinformatics e-book for beginners.
training
handbook
updated 6 months ago by
Biostar
3.4k • written 8.3 years ago by
Istvan Albert
102k
0
votes
0
replies
47
views
Defining Sex Chromosomes in PLINK for a Camel Genome
plink
vcf
updated 8 hours ago by
GenoMax
150k • written 8 hours ago by
Smilesky
• 0
0
votes
0
replies
48
views
Annotation of CpG, DMRs and DMPs with MethylKit and Genomation in R
dmp
methylkit
dmr
CpG
9 hours ago by
egascon
▴ 60
0
votes
4
replies
182
views
Depth in Cram file does not match depth in VCF file
IGV
of
Depth
SNV
coverage
updated 3 hours ago by
Jeremy Leipzig
23k • written 23 hours ago by
shu8
• 0
1
vote
0
replies
133
views
Tool:
A rust binding for pairwise/multiple sequence aligner: bsalign-rs
bsalign
alignment
1 day ago by
dwpeng
▴ 120
0
votes
0
replies
108
views
what database should I use for de novo genome in AUGUSTUS
genome
AUGUSTUS
galaxy
annotation
1 day ago by
Jl
• 0
1
vote
3
replies
619
views
Should I process two complete sets of 10x single-cell multiomics sequencing files from one donor together or separately?
10x_multiome
ENCODE
cellranger_arc
updated 8 hours ago by
GenoMax
150k • written 1 day ago by
Wu-Sheng Zhang
• 0
0
votes
2
replies
208
views
Perform DEGs with pyDESeq2 in CCLE Melanoma Data But Contains Negative Values
DEG
CCLE
Melanoma
pyDESeq2
2 days ago by
mete.han.celebi
• 0
0
votes
0
replies
136
views
energy minimisation
Energy
updated 2 days ago by
Pierre Lindenbaum
165k • written 2 days ago by
swarnadurga66666m
• 0
0
votes
1
reply
160
views
How to concatenate different domains in the target database identified by hmmsearch
multiple
domains
hmmsearch
updated 2 days ago by
GenoMax
150k • written 2 days ago by
Yongjie Zhang
▴ 110
0
votes
1
reply
168
views
How to filter Hmmsearch alignment
Hmmsearch
updated 2 days ago by
Mensur Dlakic
★ 29k • written 2 days ago by
Yongjie Zhang
▴ 110
2
votes
3
replies
2.3k
views
Defining residues as buried or exposed based in ASAs/RSAs
structural-bioinformatics
protein-biology
updated 2 days ago by
Dan A
• 0 • written 3.7 years ago by
Agenor Neto
▴ 10
1
vote
1
reply
189
views
Understanding Re-use of Query Sequences in Long Read Alignments
Alignment
PacBio
pbmm2
Minimap2
updated 2 days ago by
LauferVA
4.6k • written 2 days ago by
Charles-Alexandre Roy
▴ 50
0
votes
0
replies
126
views
Question about `vg construct`
vg
2 days ago by
zhengluo
• 0
0
votes
2
replies
248
views
Discrepancy between BAM and vcf
discrepancy
vcf
DRAGEN
updated 3 days ago by
Istvan Albert
102k • written 3 days ago by
louis-gil
• 0
2
votes
1
reply
378
views
Question about `vg giraffe`
vg
updated 3 days ago by
GenoMax
150k • written 4 days ago by
zhengluo
• 0
0
votes
1
reply
459
views
Difference in mapping rate when aligned with mapper.pl of miReep2 and bowtie1
small-RNASeq
miRNASeq
bowtie
miRDeep2
updated 3 days ago by
Ram
45k • written 9 months ago by
MIKA
• 0
3
votes
3
replies
309
views
How to align library of highly similar sequences
MPRA
variant
alignment
BWA
SNV
1 hour ago by
rustyshackleford
• 0
0
votes
3
replies
1.5k
views
How to introduce normalized and scaled seurat data into monocle 3?
Monocle
scRNA-seq
Seurat
updated 3 days ago by
Bastien Hervé
6.2k • written 22 months ago by
Sun
• 0
0
votes
1
reply
211
views
Reannotation of complete bacterial genome sequence
re-annotation
genome
whole
Automated
updated 12 hours ago by
Juke34
9.2k • written 4 days ago by
csag6433
• 0
0
votes
2
replies
765
views
What are the best tools for quantifying allele-specific expression from bulk RNA-seq data these days?
ase
phASER
allele
gene
gatk
updated 3 days ago by
GenoMax
150k • written 4 days ago by
Paulo
• 0
3
votes
4
replies
3.2k
views
Lift over of GWAS summary stat file from Hg38 to Hg19
Hg38
linux
Liftover
GWAS
Hg19
updated 3 days ago by
Mllepnos
• 0 • written 3.8 years ago by
AVA
▴ 40
8
votes
3
replies
335
views
How do I install Terminal on Windows?
Terminal
updated 4 days ago by
5heikki
11k • written 4 days ago by
phyms
• 0
1
vote
1
reply
214
views
Mapping reversion mutations
NGS
reversion
mutation
updated 4 days ago by
Ram
45k • written 4 days ago by
reddyraghuveer664
• 0
0
votes
0
replies
175
views
Issue with Fetching Population Allele Frequency in gnomAD GraphQL API
allelle
frequency
api
GraphQL
gnomad
4 days ago by
DareDevil
★ 4.4k
0
votes
1
reply
216
views
Attempts to demultiplex long reads from .pod5 result in unclassified reads
dorado
sequencing
Long-read
demultiplex
updated 4 days ago by
GenoMax
150k • written 4 days ago by
Placeholder@12654926
• 0
0
votes
1
reply
242
views
Issue with BSgenomeForge::forgeBSgenomeDataPkgFromNCBI
R
BSgenomeForge
BSgenome
NCBI
updated 4 days ago by
ATpoint
87k • written 4 days ago by
noodle
▴ 650
1
vote
1
reply
253
views
Batch effect or biological difference
Batch-effect
updated 4 days ago by
Ram
45k • written 4 days ago by
cynthier
• 0
3
votes
2
replies
296
views
Inconsistency between VCF and HGVS
VEP
4 days ago by
Senanu
▴ 30
0
votes
0
replies
184
views
VCFtools + easySFS snp # discordance
RADtags
vcftools
updated 4 days ago by
GenoMax
150k • written 4 days ago by
laurasachica7
• 0
0
votes
4
replies
3.2k
views
Generating Multiple Alignment Format file (Maf)
maf
multiple-alignment
mafft
updated 5 days ago by
Ram
45k • written 8.3 years ago by
roz_safavi
• 0
3
votes
6
replies
447
views
STAR: Paired alignment gets ~18% unmapped (too short), but single reads get >90% mapping
paired-end
star
rnaseq
4 days ago by
Davor
• 0
1
vote
0
replies
285
views
Job:
bioinformatician position, Ohio State University, Columbus, USA
bioinformatician
updated 5 days ago by
GenoMax
150k • written 5 days ago by
ilaria.palmisano
▴ 10
3
votes
4
replies
359
views
Coverage drop at assembly ends
bowtie2
bw-mem
alignment
minimap2
updated 5 days ago by
GenoMax
150k • written 5 days ago by
alenew.am
▴ 10
1
vote
4
replies
429
views
How to identify additional SNPs on EPICv2
SNPs
methylation
EPIC
5 days ago by
Basti
★ 2.0k
0
votes
1
reply
249
views
jcvi:ValueError: A total of 0 anchor was found. Aborted.
jcvi
Collinearity
updated 5 days ago by
Ram
45k • written 5 days ago by
JieQY
• 0
0
votes
5
replies
475
views
SNPcheck alternative
SNPCheck
updated 5 days ago by
GenoMax
150k • written 6 days ago by
KirGen
▴ 30
0
votes
0
replies
199
views
Comparing HMMER vs. HHrepID for LRR Repeat Detection and Boundary Consistency
hmmer
repeats
5 days ago by
jllPons
• 0
0
votes
0
replies
236
views
circos plot help
visualization
SNV
genomics
circos
updated 4 days ago by
Ram
45k • written 5 days ago by
Genomancer
• 0
2
votes
3
replies
341
views
DAVID List Upload/Gene Conversion Tool
DAVID
updated 5 days ago by
Ram
45k • written 6 days ago by
b5708137
• 0
0
votes
8
replies
3.0k
views
6 follow
CIBERSORTx error: 'x' must be an array of at least two dimensions
matrix
CIBERSORTx
signature
updated 5 days ago by
GenoMax
150k • written 2.7 years ago by
Jie
• 0
1
vote
2
replies
611
views
Can I combine segment results from Sequenza and cnvkit?
CNV
sequenza
cnvkit
6 days ago by
Ram
45k
1
vote
6
replies
491
views
Shotgun dataset with polyG, low qualiity
shotgun-data
updated 4 days ago by
andres.firrincieli
3.9k • written 6 days ago by
shevch2009
• 0
0
votes
2
replies
308
views
MAGeCK: Doing two sided test on gene level?
MAGeCK
6 days ago by
gernophil
▴ 120
0
votes
5
replies
425
views
Is TETranscripts useful for repetitive sequences analysis?
TEtranscripts
updated 5 days ago by
Michael
55k • written 6 days ago by
frarodmar17
• 0
120,856 results • Page
1 of 2418
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Answer: How to align library of highly similar sequences
Answer: How to align library of highly similar sequences
Answer: Selecting Random Pairs From Fastq?
Good QC Package (RSeQC or alternative) for Salmon mapping/quantification results?
Comment: rRNA depletion of RIP-seq samples
Comment: rRNA depletion of RIP-seq samples
Comment: rRNA depletion of RIP-seq samples
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Comment: Good QC Package (RSeQC or alternative) for Salmon mapping/quantification results
by
squidqid
• 0
MultiQC is very helpful. From my understanding, however, MultiQC will not parse the information from useful auxiliary outputs like .../aux_…
Comment: How to align library of highly similar sequences
by
rustyshackleford
• 0
Thanks so much!
Comment: Remove batch effect RnaSeq (RUVg)
by
ATpoint
87k
It is generally expected in communities like this that you read the manual first which covers this https://bioconductor.org/packages/releas…
Comment: Good QC Package (RSeQC or alternative) for Salmon mapping/quantification results
by
GenoMax
150k
If you are using single cell data then you really should be using `alevin-fry/simpleaf` packages ([**LINK**][1]) instead of `salmon`. There…
Comment: Depth in Cram file does not match depth in VCF file
by
Jeremy Leipzig
23k
can you show us that line in the VCF: `cat PC_1-7.wf_snp_clinvar.vcf | grep 69078931`
Comment: Lacking exons in gtf file of a virus' genome
by
GenoMax
150k
Since the host is always going to be there you can do the mapping to a combined three transcriptome pool, as a test/first-pass. That will g…
Comment: Good QC Package (RSeQC or alternative) for Salmon mapping/quantification results
by
squidqid
• 0
Did you ever get an answer to this question?
Comment: Lacking exons in gtf file of a virus' genome
by
ZuelTech
• 0
I mean, can I map all lists of the libraries ( HAZV-infected, LGTV-infected, uninfected) to the HAZV genome? Can I also map them to the LGT…
Comment: Lacking exons in gtf file of a virus' genome
by
GenoMax
150k
> Then, I want to map the libraries to each of the virus genome. Is this possible? Possible but will that be logical. If you have all thr…
Comment: Lacking exons in gtf file of a virus' genome
by
ZuelTech
• 0
Thank you! Btw, going back to mapping, I’m working with a vector infected with HAZV and LGTV virus. I have libraries with the host infected…
Comment: Lacking exons in gtf file of a virus' genome
by
Michael
55k
Yes, that you can do as well. Then you don't need to use STAR. But I would add the host transcriptome as a decoy there.
Comment: Lacking exons in gtf file of a virus' genome
by
ZuelTech
• 0
I see, thank you so much! I’ll try this method. Can I also use Salmon in quantification?
Comment: Lacking exons in gtf file of a virus' genome
by
Michael
55k
> Does it mean I don’t need to put a gtf file when I do mapping to HAZV > genome? Indeed, unless you want to generate the count file in ST…
Comment: Lacking exons in gtf file of a virus' genome
by
ZuelTech
• 0
Thank you! I’m new to bioinformatics, particularly on working with virus. What can you recommend on what to do with mapping with the reads …
Comment: Lacking exons in gtf file of a virus' genome
by
ZuelTech
• 0
Thank you. But I think the one you provided is from Refseq (as evident from the file name “GCF…”, mine I used the genome from GenBank with …
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