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comments
3
votes
4
replies
1.0k
views
How to remove a gene before making a cellranger reference?
fasta
gtf
cellranger
scRNAseq
updated 22 months ago by
ATpoint
86k • written 22 months ago by
bioinfo
▴ 150
0
votes
2
replies
1.0k
views
Comment:
Comment: How to remove a gene before making a cellranger reference?
22 months ago by
bioinfo
▴ 150
0
votes
0
replies
630
views
Comment:
Comment: Do I need to merge files after downloading them from SRA?
22 months ago by
bioinfo
▴ 150
1
vote
2
replies
630
views
Do I need to merge files after downloading them from SRA?
rna-seq
sra
22 months ago by
bioinfo
▴ 150
15
votes
8
replies
6.2k
views
Encountering error when loading scanpy?
scanpy
single-cell
scRNA-seq
22 months ago by
bioinfo
▴ 150
0
votes
0
replies
2.6k
views
Comment:
Comment: How to make a UMAP for single cell data and color cells by average expression of
22 months ago by
bioinfo
▴ 150
0
votes
0
replies
1.4k
views
Comment:
Comment: Problem with tximport and plasmodium falciparum
22 months ago by
bioinfo
▴ 150
0
votes
1
reply
1.4k
views
Comment:
Comment: Problem with tximport and plasmodium falciparum
22 months ago by
bioinfo
▴ 150
1
vote
0
replies
543
views
What is the difference between gseapy.prerank and gseapy.gsea?
gseapy
gsea
22 months ago by
bioinfo
▴ 150
3
votes
2
replies
685
views
Do I need to correct for sequencing depth in bulk RNA seq?
kallisto
RNA-seq
updated 22 months ago by
Ram
44k • written 23 months ago by
bioinfo
▴ 150
0
votes
0
replies
638
views
How to adjust graph in scanpy?
scanpy
single-cell
RNA-seq
23 months ago by
bioinfo
▴ 150
0
votes
0
replies
796
views
How to analyze scRNA seq data with time points (Seurat/Pseudotime)?
monocle3
scRNA-seq
pseudotime
Seurat
R
23 months ago by
bioinfo
▴ 150
0
votes
0
replies
1.4k
views
Comment:
Comment: How to perform scRNA seq analysis?
23 months ago by
bioinfo
▴ 150
2
votes
2
replies
1.4k
views
How to perform scRNA seq analysis?
single-cell
pseudobulk
seurat
23 months ago by
bioinfo
▴ 150
0
votes
0
replies
1.6k
views
Comment:
Comment: Issues with chromosome file in kallisto
24 months ago by
bioinfo
▴ 150
0
votes
1
reply
1.6k
views
Comment:
Comment: Issues with chromosome file in kallisto
2.0 years ago by
bioinfo
▴ 150
0
votes
0
replies
916
views
Comment:
Comment: Does the bootstrap in kallisto affect the BAM files?
2.0 years ago by
bioinfo
▴ 150
3
votes
2
replies
916
views
Does the bootstrap in kallisto affect the BAM files?
RNAseq
kallisto
BAM
2.0 years ago by
bioinfo
▴ 150
1
vote
4
replies
1.6k
views
Issues with chromosome file in kallisto
kallisto
updated 2.0 years ago by
dsull
★ 7.0k • written 2.0 years ago by
bioinfo
▴ 150
1
vote
2
replies
1.0k
views
How to run UMAP on single cell results from 2 different runs?
single-cell
seurat
cellranger
updated 2.0 years ago by
LChart
4.7k • written 2.0 years ago by
bioinfo
▴ 150
0
votes
1
reply
901
views
Comment:
Comment: How to check gene properties (eg GC, repetitiveness, average expression level)?
2.1 years ago by
bioinfo
▴ 150
0
votes
1
reply
1.6k
views
Comment:
Comment: Can I demultiplex run based on i5 sequence?
2.1 years ago by
bioinfo
▴ 150
0
votes
1
reply
1.6k
views
Comment:
Comment: Can I demultiplex run based on i5 sequence?
2.1 years ago by
bioinfo
▴ 150
1
vote
3
replies
901
views
How to check gene properties (eg GC, repetitiveness, average expression level)?
deseq2
kallisto
RNA-seq
updated 22 months ago by
Ram
44k • written 2.1 years ago by
bioinfo
▴ 150
0
votes
1
reply
1.6k
views
Comment:
Comment: Can I demultiplex run based on i5 sequence?
2.1 years ago by
bioinfo
▴ 150
0
votes
1
reply
1.6k
views
Comment:
Comment: Can I demultiplex run based on i5 sequence?
2.1 years ago by
bioinfo
▴ 150
0
votes
1
reply
1.6k
views
Comment:
Comment: Can I demultiplex run based on i5 sequence?
2.1 years ago by
bioinfo
▴ 150
0
votes
10
replies
1.6k
views
Can I demultiplex run based on i5 sequence?
demultiplexing
bcl2fastq
updated 2.1 years ago by
GenoMax
148k • written 2.1 years ago by
bioinfo
▴ 150
0
votes
1
reply
4.1k
views
Comment:
Comment: How to choose parameters for kallisto single end mode?
2.2 years ago by
bioinfo
▴ 150
3
votes
13
replies
4.1k
views
How to choose parameters for kallisto single end mode?
kallisto
RNA-seq
updated 19 months ago by
Istvan Albert
102k • written 2.2 years ago by
bioinfo
▴ 150
0
votes
0
replies
4.1k
views
Comment:
Comment: How to choose parameters for kallisto single end mode?
2.2 years ago by
bioinfo
▴ 150
7
votes
3
replies
2.7k
views
Should I merge my technical replicates for bulk RNA seq?
RNA-seq
DESeq2
updated 22 months ago by
Ram
44k • written 2.2 years ago by
bioinfo
▴ 150
4
votes
4
replies
1.3k
views
Can I perform DE analysis with Seurat if I only have one sample per condition?
single
RNA
Seq
cell
seurat
updated 2.2 years ago by
LChart
4.7k • written 2.2 years ago by
bioinfo
▴ 150
0
votes
0
replies
444
views
How do I get estimated gene counts for a reference that contains GFP using Kallisto?
gene
ensembl
tximport
kallisto
2.3 years ago by
bioinfo
▴ 150
0
votes
0
replies
1.6k
views
Comment:
Comment: How can I confirm that I installed bcl2fastq correctly?
2.3 years ago by
bioinfo
▴ 150
0
votes
0
replies
1.6k
views
Comment:
Comment: How can I confirm that I installed bcl2fastq correctly?
2.3 years ago by
bioinfo
▴ 150
4
votes
4
replies
1.6k
views
How can I confirm that I installed bcl2fastq correctly?
bcl2fastq
linux
ubuntu
2.3 years ago by
bioinfo
▴ 150
3
votes
2
replies
758
views
Why do I get a positive gene expression with qPCR for one my genes but in with when doing bulk RNA seq I get a count of 0?
gene
qpcr
kallisto
seq
expression
rna
updated 2.4 years ago by
dsull
★ 7.0k • written 2.4 years ago by
bioinfo
▴ 150
1
vote
3
replies
936
views
How to filter .fa file by gene_biotype
rna-seq
kallisto
updated 2.5 years ago by
Ram
44k • written 2.6 years ago by
bioinfo
▴ 150
0
votes
1
reply
1.5k
views
Comment:
Comment: How to create kallisto index that does not include ribosomal genes
2.6 years ago by
bioinfo
▴ 150
0
votes
1
reply
1.5k
views
Comment:
Comment: How to create kallisto index that does not include ribosomal genes
2.6 years ago by
bioinfo
▴ 150
0
votes
7
replies
1.5k
views
6 follow
How to create kallisto index that does not include ribosomal genes
enseml
kallisto
RNA-seq
updated 2.0 years ago by
Ram
44k • written 2.6 years ago by
bioinfo
▴ 150
0
votes
0
replies
429
views
Replicates in sc-RNA seq have different amount of cells-Can I still use them for the analysis?
Cell
seurat
Single
updated 2.6 years ago by
ATpoint
86k • written 2.6 years ago by
bioinfo
▴ 150
0
votes
2
replies
1.2k
views
Comment:
Comment: Can I use the transcripts_to_genes.txt file from kallisto for bulk RNA seq data?
2.7 years ago by
bioinfo
▴ 150
2
votes
4
replies
1.2k
views
Can I use the transcripts_to_genes.txt file from kallisto for bulk RNA seq data?
kallisto
updated 2.7 years ago by
Ram
44k • written 2.7 years ago by
bioinfo
▴ 150
0
votes
1
reply
1.4k
views
Comment:
Comment: Ensembl version for mapping and annotating genes
2.7 years ago by
bioinfo
▴ 150
3
votes
3
replies
1.4k
views
Ensembl version for mapping and annotating genes
rna-seq
kallisto
updated 2.5 years ago by
Ram
44k • written 2.7 years ago by
bioinfo
▴ 150
6
votes
2
replies
711
views
Ensembl version
ensembl
kallisto
updated 2.0 years ago by
Ram
44k • written 2.7 years ago by
bioinfo
▴ 150
2
votes
2
replies
2.8k
views
Cellranger command for RNA velocity
cellranger
Cell
velocity
RNA
Single
updated 2.9 years ago by
Friederike
9.0k • written 2.9 years ago by
bioinfo
▴ 150
4
votes
2
replies
1.1k
views
Estimated gene counts with tximport
RNA-seq
ensembl
tximport
kallisto
updated 2.5 years ago by
Ram
44k • written 3.0 years ago by
bioinfo
▴ 150
153 results • Page
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