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Comment:
Comment: How to compute TPM normalized values for TCGA miRNA data?
20 hours ago by
swbarnes2
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0
replies
275
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Comment:
Comment: Understanding STAR output (Aligned.out.sam file)
20 hours ago by
swbarnes2
14k
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votes
0
replies
252
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Answer:
Answer: Error in checkFullRank(modelMatrix) : the model matrix is not full rank, so t
1 day ago by
swbarnes2
14k
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0
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415
views
Comment:
Comment: Differential Expression Analysis using DESeq2 in R
4 days ago by
swbarnes2
14k
0
votes
1
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228
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Answer:
Answer: Clarification regarding SAM flags "mate reverse strand" (flag 16/0x10) and "read
4 days ago by
swbarnes2
14k
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0
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415
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Comment:
Comment: Differential Expression Analysis using DESeq2 in R
4 days ago by
swbarnes2
14k
0
votes
0
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339
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Answer:
Answer: Sequence read length shorter than flow cell specification
12 days ago by
swbarnes2
14k
1
vote
0
replies
709
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Comment:
Comment: PCA plot (Suggestions Needed)
12 days ago by
swbarnes2
14k
0
votes
1
reply
912
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Comment:
Comment: Adding CB tag to bam file
13 days ago by
swbarnes2
14k
1
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0
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709
views
Comment:
Comment: PCA plot (Suggestions Needed)
13 days ago by
swbarnes2
14k
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0
replies
709
views
Comment:
Comment: PCA plot (Suggestions Needed)
13 days ago by
swbarnes2
14k
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1
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558
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Comment:
Comment: To get p-values for the TPM
13 days ago by
swbarnes2
14k
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367
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Comment:
Comment: Should I use unpaired reads from trimmomatic
14 days ago by
swbarnes2
14k
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0
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298
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Answer:
Answer: from row count to tpm
15 days ago by
swbarnes2
14k
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1
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502
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Comment:
Comment: Marking duplicates using UMIs
16 days ago by
swbarnes2
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0
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0
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532
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Comment:
Comment: Free/open source 23andme-like analysis
17 days ago by
swbarnes2
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0
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1.3k
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Comment:
Comment: Cellranger count error: Input FASTQ file ended prematurely
17 days ago by
swbarnes2
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1
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912
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Comment:
Comment: Adding CB tag to bam file
17 days ago by
swbarnes2
14k
0
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0
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779
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Comment:
Comment: RNA seq differential expression analysis
19 days ago by
swbarnes2
14k
0
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1
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912
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Answer:
Answer: Adding CB tag to bam file
19 days ago by
swbarnes2
14k
2
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1
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692
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Answer:
Answer: DEG analysis of RNA-seq data across multiple tissues and two conditions
20 days ago by
swbarnes2
14k
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0
replies
611
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Answer:
Answer: Removing adapter/primer sequence from reads while keeping flanking regions
5 weeks ago by
swbarnes2
14k
0
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1
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611
views
Comment:
Comment: Removing adapter/primer sequence from reads while keeping flanking regions
5 weeks ago by
swbarnes2
14k
0
votes
0
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436
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Comment:
Comment: Seeking Help with Unexpected MA Plot Output
5 weeks ago by
swbarnes2
14k
1
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0
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430
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Comment:
Comment: Problem generating paired end reads when converting Cell Ranger's BAM result to
5 weeks ago by
swbarnes2
14k
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0
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332
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Answer:
Answer: Find out shared differentially expressed genes between two cell lines due to kno
6 weeks ago by
swbarnes2
14k
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0
replies
305
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Answer:
Answer: Detecting viruses in human rna seq data
7 weeks ago by
swbarnes2
14k
1
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1
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421
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Answer:
Answer: Is it better to pool samples prior to running deseq2, or should the algorithm be
7 weeks ago by
swbarnes2
14k
2
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571
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Answer:
Answer: Overriding a color palette in R to emphasize specific data points
7 weeks ago by
swbarnes2
14k
0
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0
replies
578
views
Comment:
Comment: RNA_seq
7 weeks ago by
swbarnes2
14k
0
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1
reply
578
views
Answer:
Answer: RNA_seq
7 weeks ago by
swbarnes2
14k
1
vote
0
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373
views
Answer:
Answer: Is it feasible to merge Illumina RNA-Seq data with varying read lengths?
8 weeks ago by
swbarnes2
14k
2
votes
0
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389
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Answer:
Answer: Re. Batch effect correction in data with a design matrix without full rank
8 weeks ago by
swbarnes2
14k
0
votes
1
reply
551
views
Answer:
Answer: RNA Seq Sample Outlier
8 weeks ago by
swbarnes2
14k
1
vote
1
reply
419
views
Answer:
Answer: STAR alignment setting for RNA-Seq data from mouse background expressing a human
8 weeks ago by
swbarnes2
14k
1
vote
1
reply
469
views
Answer:
Answer: (STAR) What causes a high number of reads that are unmapped because they are too
8 weeks ago by
swbarnes2
14k
2
votes
1
reply
317
views
Answer:
Answer: Single cell rna sequencing analysis using R help
9 weeks ago by
swbarnes2
14k
3
votes
0
replies
432
views
Answer:
Answer: single cell rna seq-how to DEG analyze??(full code & Dimplot added)
9 weeks ago by
swbarnes2
14k
1
vote
0
replies
254
views
Answer:
Answer: biological replicate in STAR command
10 weeks ago by
swbarnes2
14k
1
vote
0
replies
398
views
Answer:
Answer: PCA plot analysis
10 weeks ago by
swbarnes2
14k
0
votes
1
reply
453
views
Answer:
Answer: ENA fastq file shown as single-end but has 2 fastq files: X.fastq.gz and Y.fastq
11 weeks ago by
swbarnes2
14k
2
votes
0
replies
325
views
Comment:
Comment: Getting less DE genes when incorporating all samples
11 weeks ago by
swbarnes2
14k
1
vote
0
replies
464
views
Comment:
Comment: Count table statistical analysis
11 weeks ago by
swbarnes2
14k
1
vote
1
reply
587
views
Comment:
Comment: Trouble Understanding DESeq2 Design Formula ("Model Matrix is not Full Rank")
3 months ago by
swbarnes2
14k
0
votes
0
replies
587
views
Answer:
Answer: Trouble Understanding DESeq2 Design Formula ("Model Matrix is not Full Rank")
3 months ago by
swbarnes2
14k
1
vote
0
replies
600
views
Comment:
Comment: Three fastq files
3 months ago by
swbarnes2
14k
0
votes
0
replies
505
views
Comment:
Comment: Differential gene expression design using Deseq2
3 months ago by
swbarnes2
14k
0
votes
1
reply
1.1k
views
Comment:
Comment: Expression analysis of LncRNA from RNA-seq data
3 months ago by
swbarnes2
14k
0
votes
1
reply
1.1k
views
Comment:
Comment: Expression analysis of LncRNA from RNA-seq data
3 months ago by
swbarnes2
14k
0
votes
0
replies
576
views
Answer:
Answer: Help interpreting Samtools Flagstat results
3 months ago by
swbarnes2
14k
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