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comments
0
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0
replies
623
views
Comment:
Comment: How to handle RNA seq data with unbalanced % rRNA by group?
5 weeks ago by
ev97
▴ 40
0
votes
0
replies
623
views
Comment:
Comment: How to handle RNA seq data with unbalanced % rRNA by group?
5 weeks ago by
ev97
▴ 40
0
votes
1
reply
623
views
Comment:
Comment: How to handle RNA seq data with unbalanced % rRNA by group?
5 weeks ago by
ev97
▴ 40
0
votes
2
replies
623
views
Comment:
Comment: How to handle RNA seq data with unbalanced % rRNA by group?
5 weeks ago by
ev97
▴ 40
9
votes
10
replies
623
views
How to handle RNA seq data with unbalanced % rRNA by group?
fastqscreen
rRNA
DESeq2
RNAseq
DGE
updated 4 weeks ago by
Carlo Yague
8.9k • written 5 weeks ago by
ev97
▴ 40
0
votes
0
replies
3.3k
views
Answer:
Answer: Remove column from Seurat metadata
3 months ago by
ev97
▴ 40
0
votes
0
replies
237
views
Should I focus on genes with MGI symbols when they have several Ensembl/Entrez IDs?
biomart
entrez
mgi
Ensembl
ncbi
4 months ago by
ev97
▴ 40
0
votes
0
replies
744
views
Comment:
Comment: Should I normalize and scale my data again after doing subset(seu, idents=" ") i
5 months ago by
ev97
▴ 40
0
votes
2
replies
744
views
Should I normalize and scale my data again after doing subset(seu, idents=" ") in Seurat?
normalization
seurat
scRNA-seq
5 months ago by
ev97
▴ 40
2
votes
3
replies
1.0k
views
Is it possible to modify the gene names from the x-axis in Seurat::Dotplot?
seurat
scRNA-seq
single-cell
dotplot
updated 5 months ago by
Ram
44k • written 5 months ago by
ev97
▴ 40
0
votes
0
replies
1.0k
views
Comment:
Comment: Is it possible to modify the gene names from the x-axis in Seurat::Dotplot?
5 months ago by
ev97
▴ 40
0
votes
0
replies
1.0k
views
Comment:
Comment: Is it possible to modify the gene names from the x-axis in Seurat::Dotplot?
5 months ago by
ev97
▴ 40
0
votes
0
replies
477
views
Comment:
Comment: Is there any minimum number of counts in scRNAseq to consider that a gene is exp
5 months ago by
ev97
▴ 40
3
votes
2
replies
477
views
Is there any minimum number of counts in scRNAseq to consider that a gene is expressed?
scRNAseq
single-cell
seurat
scanpy
UMI
5 months ago by
ev97
▴ 40
0
votes
0
replies
239
views
Is it possible to keep gene_id and gene_name columns using Seurat::Read10X?
Seurat
single-cell
scRNA-seq
updated 6 months ago by
Ram
44k • written 6 months ago by
ev97
▴ 40
0
votes
0
replies
647
views
Comment:
Comment: Can 5' and 3' scRNAseq be processed with the same pipeline?
6 months ago by
ev97
▴ 40
0
votes
1
reply
647
views
Comment:
Comment: Can 5' and 3' scRNAseq be processed with the same pipeline?
6 months ago by
ev97
▴ 40
2
votes
4
replies
647
views
Can 5' and 3' scRNAseq be processed with the same pipeline?
singlecell
seurat
10XGenomics
scRNAseq
updated 6 months ago by
yura.grabovska
▴ 660 • written 6 months ago by
ev97
▴ 40
2
votes
2
replies
472
views
Removing adapters in all the RNAseq samples regardless if they have or not, is it correct?
fastqc
trimming
adapters
cutadapt
RNAseq
updated 7 months ago by
dsull
★ 6.9k • written 7 months ago by
ev97
▴ 40
0
votes
0
replies
1.1k
views
Comment:
Comment: Does removal of contaminants in RNAseq data have an impact on downstream analyse
8 months ago by
ev97
▴ 40
0
votes
1
reply
1.1k
views
Comment:
Comment: Does removal of contaminants in RNAseq data have an impact on downstream analyse
8 months ago by
ev97
▴ 40
0
votes
1
reply
1.1k
views
Comment:
Comment: Does removal of contaminants in RNAseq data have an impact on downstream analyse
8 months ago by
ev97
▴ 40
2
votes
7
replies
1.1k
views
Does removal of contaminants in RNAseq data have an impact on downstream analyses?
RNA-seq
contamination
updated 8 months ago by
Chris Dean
▴ 420 • written 8 months ago by
ev97
▴ 40
1
vote
0
replies
905
views
Comment:
Comment: 1 Bioinformatician position in San Sebastian (Spain)
9 months ago by
ev97
▴ 40
1
vote
3
replies
905
views
Job:
1 Bioinformatician position in San Sebastian (Spain)
postdoc
phd
proteomics
scRNAseq
transcriptomics
9 months ago by
ev97
▴ 40
0
votes
0
replies
791
views
Comment:
Comment: Why do I get DE non-polyadenylated genes in a RNAseq with PoliA library?
10 months ago by
ev97
▴ 40
0
votes
1
reply
791
views
Comment:
Comment: Why do I get DE non-polyadenylated genes in a RNAseq with PoliA library?
10 months ago by
ev97
▴ 40
2
votes
5
replies
791
views
Why do I get DE non-polyadenylated genes in a RNAseq with PoliA library?
lncRNA
PoliA
DGE
RNAseq
DESeq2
updated 10 months ago by
i.sudbery
20k • written 10 months ago by
ev97
▴ 40
0
votes
1
reply
548
views
Comment:
Comment: What do you usually use for QC using DNBseq?
10 months ago by
ev97
▴ 40
1
vote
3
replies
548
views
What do you usually use for QC using DNBseq?
DNBseq
fastqc
qc
Illumina
RNA-seq
updated 10 months ago by
GenoMax
147k • written 10 months ago by
ev97
▴ 40
0
votes
0
replies
577
views
Job:
PhD position at CiMUS (Santiago de Compostela, Spain)
transcriptomics
PhD
RNA-seq
Single-Cell
updated 11 months ago by
Ram
44k • written 11 months ago by
ev97
▴ 40
0
votes
0
replies
324
views
Having different % aligned reads in RPF and mRNA for TE analysis
RPF
Ribo-Seq
Translation
TE
RNA-seq
11 months ago by
ev97
▴ 40
0
votes
0
replies
1.8k
views
Comment:
Comment: DNBseq vs Illumina
16 months ago by
ev97
▴ 40
6
votes
5
replies
1.8k
views
DNBseq vs Illumina
DNBseq
fastq
sequencing
Illumina
RNA-seq
16 months ago by
ev97
▴ 40
0
votes
0
replies
1.8k
views
Comment:
Comment: DNBseq vs Illumina
16 months ago by
ev97
▴ 40
2
votes
2
replies
725
views
Are SAM attributes necessary if I only keep the BAM files using STAR?
BAM
SAM
RNA-seq
STAR
updated 16 months ago by
GenoMax
147k • written 16 months ago by
ev97
▴ 40
0
votes
1
reply
701
views
Comment:
Comment: How to check if the total million of reads that I have are the ones that I bough
16 months ago by
ev97
▴ 40
2
votes
3
replies
701
views
How to check if the total million of reads that I have are the ones that I bought?
fastq
RNA-seq
updated 16 months ago by
Ram
44k • written 16 months ago by
ev97
▴ 40
0
votes
1
reply
1.2k
views
Comment:
Comment: Same GEO Accession, different SRR number, how to download this RNA-seq paired-en
17 months ago by
ev97
▴ 40
2
votes
3
replies
1.2k
views
Same GEO Accession, different SRR number, how to download this RNA-seq paired-end data?
sra-toolkit
fastq
RNA-seq
SRA
updated 17 months ago by
GenoMax
147k • written 17 months ago by
ev97
▴ 40
0
votes
0
replies
2.9k
views
Comment:
Comment: Negative values after batch correction using removeBatchEffect from Limma
2.3 years ago by
ev97
▴ 40
0
votes
0
replies
439
views
Adjustment by several variables, sequential adjustment or combine them in a single one?
rnaseq
combat-seq
batch
removeBatchEffect
limma
2.3 years ago by
ev97
▴ 40
0
votes
0
replies
570
views
How to choose the biological variables if you want to keep all the biological signal from your data when you have to adjust by batch?
rnaseq
combat-seq
batch
removeBatchEffect
limma
2.3 years ago by
ev97
▴ 40
2
votes
4
replies
2.9k
views
Negative values after batch correction using removeBatchEffect from Limma
batch
limma
removeBatchEffect
rna-seq
batch-effect
updated 7 months ago by
Ram
44k • written 2.3 years ago by
ev97
▴ 40
0
votes
0
replies
971
views
Comment:
Comment: What type of normalization does removeBatchEffect function require as the input?
2.3 years ago by
ev97
▴ 40
1
vote
2
replies
971
views
What type of normalization does removeBatchEffect function require as the input?
batch
limma
removeBatchEffect
rna-seq
batch-effect
updated 7 months ago by
Ram
44k • written 2.3 years ago by
ev97
▴ 40
7
votes
4
replies
1.6k
views
Is there any journal article to reference removeBatchEffect function from Limma?
batch
removebatcheffect
limma
rna-seq
batch-effect
updated 7 months ago by
Ram
44k • written 2.3 years ago by
ev97
▴ 40
0
votes
0
replies
1.6k
views
Comment:
Comment: Is there any journal article to reference removeBatchEffect function from Limma?
2.3 years ago by
ev97
▴ 40
0
votes
1
reply
3.1k
views
Comment:
Comment: How to adjust by multiple variables using ComBat-Seq?
2.3 years ago by
ev97
▴ 40
0
votes
1
reply
3.1k
views
Comment:
Comment: How to adjust by multiple variables using ComBat-Seq?
2.3 years ago by
ev97
▴ 40
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