0
votes
1
reply
46
views
0
votes
1
reply
668
views
1
vote
1
reply
162
views
2
votes
6
replies
1.1k
views
0
votes
0
replies
46
views
8 hours ago by
rene.j.erhardt
▴
20
0
votes
9
replies
220
views
1
vote
2
replies
142
views
0
votes
0
replies
74
views
8
votes
8
replies
627
views
0
votes
0
replies
80
views
0
votes
0
replies
79
views
20 hours ago by
Chris
▴
260
0
votes
1
reply
265
views
0
votes
2
replies
276
views
2
votes
3
replies
260
views
updated 22 hours ago by
Arup Ghosh
3.2k
•
written 1 day ago by
chaco001
▴
40
1
vote
3
replies
277
views
updated 15 hours ago by
Mensur Dlakic
★
27k
•
written 1 day ago by
O.rka
▴
710
1
vote
2
replies
279
views
18 hours ago by
Francesco
▴
10
Recent Votes
Recent Locations •
All
South Korea, just now
Bologna, 1 minute ago
United Kingdom, 2 minutes ago
European Union, 4 minutes ago
United States, 10 minutes ago
Israel, 11 minutes ago
India, 14 minutes ago
Recent Awards •
All
Popular Question to
husensofteng
▴
410
Popular Question to
Raheleh
▴
260
Popular Question to
Aspire
▴
300
Popular Question to
rene.j.erhardt
▴
20
Popular Question to
yueli7
▴
250
Popular Question to
a_bis
▴
40
Recent Replies
Comment: Downloading full alignments from Pfam
by
GenoMax
141k
Not as convenient but the entire set of full alignments can be downloaded here: https://ftp.ebi.ac.uk/pub/databases/Pfam/current_release/Pf…
Comment: How many reads for WGS Sequencing?
by
GenoMax
141k
> What does clipped Fastq mean?
Probably means that NCBI has already scanned and trimmed adapter sequencers.
> both forward and revers…
Comment: How many reads for WGS Sequencing?
by
GenoMax
141k
You can use the default `adapters.fa` file included in the `resources` folder of [**BBMap suite**][1] (program to use is `bbduk.sh`) or a p…
Comment: How many reads for WGS Sequencing?
by
GenoMax
141k
Only way to find out is to try. It may work but if it is not going to then you will find that out quick (process would likely crash because…
Comment: How many reads for WGS Sequencing?
by
Ruqaiya
•
0
Also, there is no mention of adapter sequence so which adapter sequence should i use to remove it from some other reads?
Comment: How many reads for WGS Sequencing?
by
Ruqaiya
•
0
Also, Do you think i can reproduce at least most part of the data from the paper just on my laptop? It has 4 logical processors (Intel(R) C…
Comment: NGS forensics: how to know if data is fabricated
by
Philipp Bayer
8.3k
While I think this is an interesting case, I've before found cleaned 'raw' data on SRA. It happens: bioinformaticians receive the raw data,…
Comment: How many reads for WGS Sequencing?
by
Ruqaiya
•
0
1. What does clipped Fastq mean? both forward and reverse reads in the same file?
2. Why would you remove 2000bp contigs? I feel it will gi…
Comment: How many reads for WGS Sequencing?
by
Ruqaiya
•
0
Well, I downloaded both the reads from-[LINK][1]. Thats all i did. Thats the only thing i need to do, right?
I'l try spades.
[1]: http…
Comment: How to assign cell types after integration in scRNA
by
Francesco
▴
10
Thanks for the fast reply.. however i had only partially understood the answer. Is correct saying that low-representation integration and b…
Answer: Can I run cellassign on samples independently if there is batch effect present?
by
Francesco
▴
10
Hi! Whenever you do make independent analysis or aggregate data and analyze them, most cell type assignment tools start from raw counts to …
Answer: ChIP-seq datasets: input samples omitted?
by
ATpoint
82k
In my hands (and from what I know based on many years here) inputs are almost exclusively used during peak calling to correct for loci-spec…
Comment: How to assign cell types after integration in scRNA
by
ATpoint
82k
No difference here. After all you typically want a label per cluster/group, so either subset to the control condition to do the assignment,…
Comment: Differential Expression using Isoseq-supplemented reference transcriptome
by
gaoanwei
•
0
I found the question and ASK the GPT4 turbo
Traffic: 1966 users visited in the last hour
Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by the
version 2.3.6
version 2.3.6