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With the `--read2-only` option (which isn't available yet on the bioconda version), this will complain about a lack of `--bc-pattern`.
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it's raw data **fastq.gz**, so for example I have 4 different samples from different tissue, sample A with 3 replicates, samples B,C and D …
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> The goal is to remove all the variants which are unique to the KWSBambina sample.
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The first example used the adapter file NexteraPE-PE.fa, but it generated more than 50% unpaired data......
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Ok so I definitely didn't understand at first what my goal was.
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What data do you have? Generally, you start with sequencing data, which you need to clean and then map to a reference genome. Then dependin…
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> when I didn't provide the adapter file
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and if I use the command as such:
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