Allele specific expression of imprinted gens from 10x scRNA-seq data
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10 days ago
singcell • 0

Hello,

I'm interested in finding expression of known imprinted genes (expressed from one allele only, monoallelic expression) within each cluster in my single-cell RNA-sequencing (scRNA-seq) data generated from 3' transcript using 10x genomics. I also wanted to identify novel expressed imprinted genes within each cluster from this data. This scRNA-seq data comes from offspring tissues generated by crossing two different parent mouse strains with known genomic single nucleotide variants/ polymorphisms (SNPs/SNVs). What is the best approach for conducting this analysis? Is there any pipeline available for this purpose?

Thank you

scrna-seq imprinted 10x genes • 150 views
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