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121,522 results • Page
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0
votes
0
replies
16
views
Incomplete assembly in HPRC dataset
pangenome
HPRC
reference
1 hour ago by
ohell
• 0
3
votes
3
replies
111
views
Joint Calling for Large Germline WGS Cohort
NGS
RAM
cohort
Genomics
WGS
updated 18 minutes ago by
Jeremy Leipzig
23k • written 7 hours ago by
j.k3096
▴ 20
0
votes
4
replies
108
views
how shoud the phred score be intepreted?
sequencing
updated 20 minutes ago by
GenoMax
152k • written 5 hours ago by
QX
▴ 70
843
votes
170
replies
181k
views
112 follow
News:
The Biostar Handbook. A bioinformatics e-book for beginners.
training
handbook
updated 10 weeks ago by
Biostar
3.6k • written 8.6 years ago by
Istvan Albert
102k
0
votes
2
replies
223
views
Inherited pipeline uses RNA-seq variant calls for WASP filtering—shouldn’t it be external SNPs?
WASP
7 hours ago by
jonas.andersson
▴ 40
0
votes
0
replies
43
views
Identifying RBP motifs overlapping exons
motifs
bedtools
RBP
7 hours ago by
RK
• 0
0
votes
0
replies
51
views
WGCNA analysis for Proteomics data
Proteomics
WGCNA
8 hours ago by
Sumit Paliwal
▴ 40
0
votes
0
replies
52
views
quality control for 16s metagenome reads
quality
metagneome
control
16s
fastp
8 hours ago by
m90
▴ 30
3
votes
8
replies
6.5k
views
7 follow
Cellranger count pipestance failed: The read lengths are incompatible with all the chemistries
10x
cellranger
single-cell
updated 9 hours ago by
zhang
• 0 • written 3.0 years ago by
firestar
★ 1.7k
0
votes
1
reply
506
views
News:
Hands-On Workshop - Next-Generation Sequencing Data Analysis: A Practical Introduction (September 10-12, 2025 in Berlin, Germany)
Workshop
RNA-Seq
Illumina
VariantCalling
DNA-Seq
9 hours ago by
ecSeq Bioinformatics
▴ 20
0
votes
0
replies
61
views
How to merge multiple pangenome graph files (generated by MC and PGGB)?
vg
pangenome
Minigraph-Cactus
PGGB
8 hours ago by
Yao
▴ 30
1
vote
0
replies
130
views
Can iHS and XP-EHH be applied to targeted sequencing data?
sequencing
targeted
ngs
ihs
2 days ago by
slzr_
▴ 10
1
vote
0
replies
135
views
Identify SNP, Indel variants from a List of FASTA sequence
SNP
2 days ago by
Trinh
▴ 10
0
votes
2
replies
222
views
PacBio amplicon reads partially aligned using minimap2 – library or analysis issue?
PacBio
minimap2
alignment
variants
pbmm2
updated 21 minutes ago by
GenoMax
152k • written 2 days ago by
louisflower1999
• 0
0
votes
0
replies
366
views
Job:
Biomedical Data Scientist / Systems Biologist / Omics Data Analyst / Bioinformatician / Computational Biologist (Tulane University, New Orleans, LA)
TUPulm
2 days ago by
yzhou40
• 0
0
votes
0
replies
189
views
Batch effect in codominant microsatellite data – how to correct
population-genetics
batch-effect
R
microsatellite
genotyping
3 days ago by
shervin
• 0
2
votes
3
replies
314
views
UCSC genome browser negative strand positions
Genomic
Data
Browser
UCSC
Visualization
Genome
updated 3 days ago by
Maximilian Haeussler
★ 1.8k • written 4 days ago by
ijarne
▴ 20
0
votes
1
reply
202
views
Error when computing bedtools bamtobed -bedpe
human
bedtools
libraries
illumina
bamtobed
updated 3 days ago by
ATpoint
88k • written 3 days ago by
María José
▴ 10
0
votes
9
replies
673
views
Ligand-Receptor analysis using LIANA - question about specificity
scRNA-seq
communication
cell-cell
updated 3 days ago by
Muhammad
▴ 10 • written 7 days ago by
abedkurdi10
▴ 190
2
votes
4
replies
1.1k
views
PRS calculation from two sample genotype results
prs
calculation
updated 3 days ago by
Muhammad
▴ 10 • written 6 months ago by
Arun
• 0
1
vote
16
replies
5.9k
views
PRS in UK Biobank - no covariate file and no phenotype file
UK
Biobank
PRS
3.8 years ago by
Jalil Sharif
▴ 80
39
votes
14
replies
2.6k
views
13 follow
Forum:
How do you estimate time to complete a project or task?
career
ngs
updated 3 days ago by
Анна
• 0 • written 5 months ago by
sviatoslav.kendall
▴ 980
0
votes
0
replies
141
views
News:
Introduction to Deep Learning for Biologists - online course, 3–7 November
MachineLearning
Python
deepLearning
ConvolutionalNeuralNetwork
3 days ago by
Physalia-courses
★ 2.6k
1
vote
2
replies
780
views
Running trajectory and velocity analysis with multiple samples
seurat
velocity
loom
trajectory
written 15 months ago by
gogeni5529
▴ 70
2
votes
6
replies
478
views
Weird p-value distribution on edgeR results
edgeR
pvalue-distribution
gene-expression
updated 3 days ago by
Gordon Smyth
★ 8.2k • written 4 days ago by
Guillermo
▴ 10
0
votes
1
reply
204
views
NMR modelling using xplor-NIH
peptide
xplor-NIH
NMR
2 days ago by
npk107
• 0
1
vote
3
replies
280
views
PCA interpretation
chip
seq
PCA
updated 3 days ago by
rfran010
★ 1.6k • written 3 days ago by
Irving
• 0
0
votes
0
replies
173
views
News:
course: Reproducibility in Bioinformatics - Only 2 seats remain!
Singularity
Git
Reproducibility
Docker
Snakemake
4 days ago by
Physalia-courses
★ 2.6k
0
votes
0
replies
168
views
Annotate phylogenetic trees with bars or strips
otu
phylogenetics
R
taxonomy
metabarcoding
4 days ago by
Luca Arbore
▴ 10
2
votes
2
replies
371
views
Identifying Long Reads Spanning Chromosomal Breakpoints
ONT
samtools
Whole-Genome
updated 4 days ago by
trausch
★ 2.0k • written 5 days ago by
Noah
▴ 10
0
votes
2
replies
294
views
PAML output
PAML
Codeml
Selection
updated 16 hours ago by
cfos4698
★ 1.1k • written 4 days ago by
Morjina
• 0
1
vote
0
replies
223
views
how to produce your own Nx plot
R
contiguity
plot
Nx
4 days ago by
Matteo Ungaro
▴ 130
1
vote
2
replies
335
views
Interpreting inconsistency between BLAST hits and phylogenetic clustering i
tree
phylogenetic
blast
4 days ago by
triplee0305
▴ 20
0
votes
0
replies
191
views
News:
Online course - Environmental Metagenomics - 13-17 October
Illumina
MAGs
Nanopore
Metagenomics
4 days ago by
Physalia-courses
★ 2.6k
0
votes
2
replies
317
views
Deg Difference in Deseq2
Deg
updated 5 days ago by
swbarnes2
15k • written 5 days ago by
j.k.owaresat
• 0
0
votes
0
replies
233
views
No gene_trans_map file in CD-HIT-EST output
cd-hit-est
Trinity
RNAseq
Trinotate
updated 5 days ago by
GenoMax
152k • written 5 days ago by
ofarinas621
• 0
0
votes
3
replies
372
views
MultiQC report - unique reads from sequence counts
fastQC
unique
reads
multiQC
4 days ago by
Rozita
▴ 40
2
votes
3
replies
352
views
NCBI Reference Genomes
genomes
ncbi
updated 5 days ago by
GenoMax
152k • written 5 days ago by
anna
▴ 70
0
votes
0
replies
216
views
GATK HaplotypeCaller missed variants in amplicon gene panel data
amplicon
gene_panel
haplotypecaller
variant_calling
gatk
5 days ago by
Sofya
• 0
0
votes
1
reply
497
views
Missing gem_classification.csv in Cell Ranger 9.0.0 Output Using cellranger multi with Combined Human-Mouse Reference
gem_classification
10x
cellranger_multi
combined_human-mouse_reference
updated 3 days ago by
GenoMax
152k • written 5 days ago by
tlallihuacan
• 0
0
votes
0
replies
203
views
Extracting logR and Baf files genome studio
Genomestudio
5 days ago by
1769mkc
★ 1.3k
0
votes
1
reply
377
views
Statistical analysis in a metagenomics study to validate biological differences
Metagenomics
analysis
updated 5 days ago by
chen
★ 2.5k • written 9 days ago by
Chijioke
• 0
1
vote
2
replies
574
views
Assembly issues
adapters
Assembly
Spades
updated 5 days ago by
chen
★ 2.5k • written 4 weeks ago by
anna
• 0
0
votes
5
replies
599
views
Trouble removing adapters from sequences using cutadapt
removal
cutadapt
adapter
primer
updated 5 days ago by
chen
★ 2.5k • written 10 days ago by
k.lagan
• 0
0
votes
5
replies
2.0k
views
Fastp
fastp
updated 5 days ago by
chen
★ 2.5k • written 3.2 years ago by
nishimalhotra2612
▴ 50
5
votes
10
replies
800
views
Need help using ExpansionHunter
ExpansionHunter
3 days ago by
brianhill_nc
• 0
0
votes
1
reply
277
views
How to extract gene list from GEO dataset in R
coding
beginner
question
r
updated 4 days ago by
manaswwm
▴ 570 • written 5 days ago by
decorus
• 0
0
votes
0
replies
246
views
WGBS Analysis with 1 control sample and 6 disease samples
one
WGBS
sample
5 days ago by
Cassandra
• 0
0
votes
0
replies
277
views
Comparing and plotting multinomial elastic net performance
ROC
elasticnet
plotting
glmnet
machinelearning
6 days ago by
bioinfo
• 0
2
votes
3
replies
436
views
How to add gene lable on GSEA plot
gseaplot
R
updated 5 days ago by
yura.grabovska
▴ 780 • written 6 days ago by
Emy Alade
▴ 20
121,522 results • Page
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Recent Votes
Joint Calling for Large Germline WGS Cohort
Creating a karyotype plot with genes using karyoploteR
Change sample names in vcf
A: Change sample names in vcf
Joint Calling for Large Germline WGS Cohort
Answer: Joint Calling for Large Germline WGS Cohort
Answer: PRS calculation from two sample genotype results
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Comment: PacBio amplicon reads partially aligned using minimap2 – library or analysis iss
by
Zev.Kronenberg
12k
@genomax has a good point about trying `pbaa`. The tool will output consensus sequences of the different alleles. You can then use MSA to a…
Answer: Joint Calling for Large Germline WGS Cohort
by
Jeremy Leipzig
23k
Not to get too pedantic but joint genotyping solves a different problem (removing artefactual variants) from producing a population VCF tha…
Answer: Joint Calling for Large Germline WGS Cohort
by
DBScan
▴ 490
Another option would be HAILs VDS Combiner, [https://hail.is/docs/0.2/vds/hail.vds.combiner.VariantDatasetCombiner.html#hail.vds.combiner.V…
Comment: how shoud the phred score be intepreted?
by
QX
▴ 70
thank you for explanation and documentation
Comment: how shoud the phred score be intepreted?
by
GenoMax
152k
Illumina sequencing assumes/generally expects that clusters in a sequencing field have an even distribution of ACTG so for every sequencing…
Comment: how shoud the phred score be intepreted?
by
QX
▴ 70
what do you mean by 'low nucleotide diversity'. If it is low nucleotide diversity, is it supposed to have consistent signals, leading to hi…
Answer: how shoud the phred score be intepreted?
by
GenoMax
152k
> Does the phred-score is affected by the position of the base in all the reads (vertical), or all the bases that belong to a single read (…
Answer: Joint Calling for Large Germline WGS Cohort
by
Pierre Lindenbaum
166k
try glnexus https://github.com/dnanexus-rnd/GLnexus/wiki/Getting-Started
Comment: Inherited pipeline uses RNA-seq variant calls for WASP filtering—shouldn’t it be
by
jonas.andersson
▴ 40
Thank you so much for you answer @iansudbery! Yes I have read it too and I don't think it's totally clear either, so I guess I might not be…
Answer: Inherited pipeline uses RNA-seq variant calls for WASP filtering—shouldn’t it be
by
i.sudbery
21k
Looking through the WASP documentation, and paper, its a little unclear. Clearly what is expected is a set of "known" SNPs. However, I'm no…
Answer: Cellranger count pipestance failed: The read lengths are incompatible with all t
by
zhang
• 0
I think the first thing is to make sure the data really comes from a 10× Genomics platform. As far as I know, Seq-Well usually has shorter …
Answer: Hands-On Workshop - Next-Generation Sequencing Data Analysis: A Practical Introd
by
ecSeq Bioinformatics
▴ 20
The course fills up quickly. If you want to take part, [apply now][1]. [1]: https://www.ecseq.com/workshops/workshop_2025-07-NGS-N…
Comment: PAML output
by
cfos4698
★ 1.1k
Also you might want to consider this paper and tool: https://onlinelibrary.wiley.com/doi/abs/10.1002/ece3.5015
Comment: how to make go term plot and kegg pathway plot using InterProScan output
by
analyst
▴ 70
Anyone please add your valuable suggestion?
Comment: PacBio amplicon reads partially aligned using minimap2 – library or analysis iss
by
GenoMax
152k
Only including this as an alternate option to try (if your reads are HiFi) : https://github.com/PacificBiosciences/pbAA > but both the 5′ …
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