Entering edit mode
4.4 years ago
singlegenome
•
0
Hello! I just got some paired-end sequencing data that has R1 demultiplexed and in separate sample-specific folders, but R2 all in a single big file. According to the sequencing provider, apparently one can use this data without demultiplexing R2 separately. How could you demultiplex R2 and match the reads to those in R1 files? They didn't provide index files either.
Can't find a similar case so I opened a new question. Is there a really easy way to do this that I'm not getting, or should I ask the sequencing provider to just demultiplex R2? Any tips appreciated!
What application are you doing? Are sequencing services really so lazy that they won't demultiplex samples? What on earth is gained by pushing that off to the end user?
Targeted sequencing to call indels. I don't think there is any advantage in doing this; they are merely lazy. Seems like the best solution is just to write them an email and ask for R2 demultiplexed.