Hi there,
I am working on the de novo analysis of bacteria. This bacteria is related to Bacillus paranthracis, which was identified using rMLST, TYGS, and BLAST analysis. The genome assembly file was then provided to the KEGG-KASS tool. Although the prokaryotic dataset was selected for analysis, the identified KO terms seem irrelevant to bacteria, such as Aging, Digestive system, Endocrine system, Immune system, Excretory system, Nervous system, Immune system, and Cellular eukaryotes.
Now, I want to perform pathway analysis either using KEGG Orthology terms (KO) as extracted from KEGG-KASS or by exploring alternative methods to utilize bacterial genome assembly or protein sequences for pathway identification. Please suggest an alternative tool or method.
