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1,000 results • Page 1 of 20
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5
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4
replies
339
views
Forum: Ideal PC configurations and operating system for bioinformatics laboratory
PC
16 hours ago by Estevão • 0
0
votes
0
replies
171
views
Forum: Improvement on automatic annotation of single-cell RNA-seq data from the literature using LLM
annotation scRNA llm
10 days ago by yxwucq • 0
4
votes
2
replies
313
views
Forum: Bioinformatics: Where do I start
Sequencing
updated 16 days ago by 43k • written 16 days ago by ▴ 10
7
votes
7
replies
1.5k
views
Forum: Bioinformatician who was retaliated against after reporting discriminatory harassment - Please help me navigate getting out of the situation I'm in
Career advice
updated 17 days ago by ★ 2.5k • written 18 days ago by • 0
1
vote
2
replies
357
views
Forum: VCF bulk handling
bcftools SnpEff WGS VEP VCF
15 days ago by Javier • 0
16
votes
10
replies
1.1k
views
6 follow
Forum: F*up Night style events for Bioinformatics ? Comment if you're interested!
mistakes
updated 4 weeks ago by 5.3k • written 5 weeks ago by ★ 2.4k
1
vote
1
reply
225
views
Forum: Can I use this community for software development?
software-development
updated 5 weeks ago by 43k • written 5 weeks ago by • 0
5
votes
2
replies
391
views
Forum: Can questions and answers on biostars be asked only in English? Can't I use Russian for example?
language
updated 5 weeks ago by 54k • written 5 weeks ago by • 0
0
votes
0
replies
178
views
Forum: Field of Study in Computational Biology
Genomics Computational-Biology PhD
updated 6 weeks ago by 43k • written 6 weeks ago by • 0
1
vote
3
replies
485
views
Forum: Development plan for a bioinformatics database developer/coder
developer
7 weeks ago by pixie@bioinfo ★ 1.5k
7
votes
8
replies
713
views
Forum: Bioinformatics career transition
career forum
updated 7 weeks ago by ★ 16k • written 7 weeks ago by ▴ 20
4
votes
2
replies
489
views
Forum: Exploring Amino Acid Patterns in Proteins Through N-gram Analysis
protein CAFA5
7 weeks ago by Anatoly A. ▴ 10
6
votes
6
replies
780
views
Forum: You have 3 days of zero obligations and expansive resources. What are you building?
hypothetical thought-experiment
8 weeks ago by jared.andrews07 ★ 16k
0
votes
0
replies
165
views
Forum: What other data types and pipelines you would like to see from the Genomics Data Commons (GDC)
gdc tcga genomics-data-commons
updated 8 weeks ago by 43k • written 8 weeks ago by ★ 1.2k
0
votes
2
replies
389
views
Forum: What's the state of the art in bacterial whole genome alignment?
whole genome alignment mauve
8 weeks ago by Dave Carlson ★ 1.7k
31
votes
12
replies
1.0k
views
7 follow
Forum: Nextflow and self-made pipelines opinion
nextflow pipeline data-analysis
9 weeks ago by karlaarz ▴ 90
5
votes
4
replies
567
views
Forum: Would reaching out to local labs and startups help with my career transition?
career volunteering
updated 10 weeks ago by 142k • written 10 weeks ago by ▴ 10
1
vote
2
replies
375
views
Forum: Exploring Career Reentry: Challenges and opportunities for Women After Maternity and Childcare Breaks
Maternity Career-Gap career-reentry
updated 10 weeks ago by 82k • written 10 weeks ago by ▴ 20
8
votes
7
replies
819
views
6 follow
Forum: Tower Server configuration for NGS data analysis
NGS
updated 10 weeks ago by ▴ 120 • written 3 months ago by • 0
1
vote
0
replies
282
views
Forum: list of bioinformatics tools in cancer genomics
mutations CNA cancer genomics LOH
updated 10 weeks ago by 161k • written 10 weeks ago by ★ 1.4k
4
votes
2
replies
382
views
Forum: bulk seq vs. sc-seq
scATAC-seq ATAC-seq scRNAseq RNAseq
updated 12 weeks ago by ★ 5.9k • written 12 weeks ago by ▴ 10
8
votes
6
replies
2.8k
views
Forum: Demonstrating Exponential Growth In Pre-School
education
written 10.2 years ago by 10k
0
votes
0
replies
205
views
Forum: tutorial for carrier screening analyses?
carrier-screening
updated 3 months ago by 43k • written 3 months ago by ▴ 20
2
votes
3
replies
437
views
Forum: My Project
Project CLI MD-Simulations Molecular-docking
3 months ago by Ajay A Shettigar • 0
17
votes
11
replies
1000
views
Forum: How to overcome the imposter syndrome in bioinformatics?
advice
3 months ago by sil_bioinfo ▴ 40
10
votes
7
replies
2.2k
views
Forum: How to learn bioinformatics?
learning
updated 3 months ago by ★ 1.3k • written 23 months ago by ▴ 20
26
votes
27
replies
2.1k
views
10 follow
Forum: Determining computer time required
computer hpc time
updated 3 months ago by ★ 5.9k • written 3 months ago by ▴ 40
7
votes
6
replies
740
views
Forum: where to work as a bioinformatician (NGS/IA)
employment omics ia ngs
updated 3 months ago by 43k • written 3 months ago by ▴ 20
21
votes
12
replies
3.5k
views
Forum: How to Use Biostars Part-3: Formatting Text and Using GitHub Gists
meta how-to documentation
3 months ago by Ram 43k
2
votes
1
reply
436
views
Forum: Cataloguing available spatial transcriptomics tools, then linking them together into a workflow?
cytassist transcriptomics nanostring xenium visium
3 months ago by LauferVA 4.2k
49
votes
20
replies
2.1k
views
10 follow
Forum: First experiences matter
first-time tolerance
updated 3 months ago by ★ 2.6k • written 4 months ago by ★ 27k
4
votes
3
replies
780
views
Forum: How to get started in bioinformatics
students
updated 3 months ago by 43k • written 4 months ago by ▴ 10
2
votes
2
replies
586
views
Forum: Do batches contain biological variations in omics data (RNAseq or scRNAseq)?
combat single-cell omics batch rnaseq
updated 4 months ago by 43k • written 4 months ago by ▴ 40
0
votes
2
replies
3.2k
views
Forum: I’m considering a career transition from software engineering to bioinformatics. Is it a good fit for me?
career
updated 4 months ago by 142k • written 4 months ago by ▴ 10
5
votes
3
replies
615
views
Forum: Binning the rare biosphere
metagenomics soil assembly techniques binning
updated 4 months ago by ★ 27k • written 4 months ago by ▴ 20
6
votes
2
replies
669
views
Forum: how to start Bioinformatics?
basics
updated 4 months ago by ★ 27k • written 4 months ago by • 0
19
votes
5
replies
1.1k
views
6 follow
Forum: What Are The Most Influential Bioinformatics/Computational Biology Papers Of 2023?
genomics snps survey papers
4 months ago by Raony Guimarães ★ 1.4k
4
votes
6
replies
932
views
6 follow
Forum: Bioinformatics: what do you think of it in the next 20 years?
future
updated 5 months ago by 43k • written 5 months ago by • 0
1
vote
1
reply
492
views
Forum: want to work in open source project/project in bioinformatics
project
updated 5 months ago by 43k • written 5 months ago by • 0
3
votes
5
replies
1.0k
views
Forum: Current Trends in Bioinformatics
structural-bioinformatics
updated 5 months ago by 43k • written 5 months ago by • 0
21
votes
11
replies
2.5k
views
6 follow
Forum: Rust bioinformatics projects ideas
projects scRNA-seq RNA-seq Rust
updated 5 months ago by 22k • written 14 months ago by ▴ 150
176
votes
46
replies
19k
views
26 follow
Forum: Crac: Funny And/Or Weird Names For Bioinformatics Tools
humor
5 months ago by Istvan Albert 100k
3
votes
2
replies
626
views
Forum: RNA-Seq data analysis - question
Python RNA-Seq R
updated 5 months ago by ▴ 10 • written 5 months ago by ▴ 40
0
votes
0
replies
372
views
Forum: How to learn bioinformatics online?
self-education education learning
updated 6 months ago by 43k • written 6 months ago by • 0
6
votes
5
replies
2.3k
views
Forum: Maintaning internal or private installation of Biostar or a similar Q&A site
Biostars
6 months ago by tshtatland ▴ 190
7
votes
9
replies
1.4k
views
6 follow
Forum: Mentor for a beginner bioinformatics student
project mentor
6 months ago by UT_FAN ▴ 40
0
votes
0
replies
461
views
Forum: Steps after doublet detection in single cell data
scRNA doublet
6 months ago by ishwarvh • 0
0
votes
0
replies
357
views
Forum: Automated Checks And QA
retrospective
6 months ago by Sasha ▴ 840
7
votes
13
replies
1.4k
views
Forum: How do you validate and verify your pipeline's software updates?
Validation Verification Quality-Assurance
updated 6 months ago by 43k • written 7 months ago by ▴ 10
21
votes
7
replies
11k
views
Forum: Bioinformatics short paper journal resource [software/webapps/database]
journal software webapp
updated 7 months ago by ▴ 100 • written 8.1 years ago by 11k
1,000 results • Page 1 of 20
Recent Votes
Answer: Two references 1. genome 2. plasmid for bowtie2
A: Iterating through paired-end reads in samtools / pysam
Answer: Where Can I Download Some Bam Files?
Download SAM/BAM files from SRA takes ages!!!
Comment: Bedtools merge minimum overlap?
Convert sra to bam file error handling
Answer: Interpreting TCGA .rsem.genes.results and .rsem.genes.normalized_results files.
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Recent Replies
Comment: HOMER on AWS by clairechung112 • 0
Hi. I guess it is solved by now, but as I did not find the answer immediately online, here is the answer I posted on a relevant question: h…
Answer: homer not configured properly by clairechung112 • 0
A late reply, but I just solved exactly the same error upon a request in the team. Please see if it helps in case anyone meets the same err…
Comment: Spike-in control found in raw reads (16S amplicon seq) but not picked up by DADA by Chris Dean ▴ 390
Was the spike-in a commercial product, e.g., from Zymo? Can you provide more information about what cells or DNA was spiked into your samp…
Comment: Bedtools merge minimum overlap? by Alex Reynolds 35k
Note that, in place of `sort-bed - | uniq -`, you can pass the `--unique` option to `sort-bed`. The input is not strictly a BED6 file, but…
Comment: Post-imputation plot by LChart 3.9k
Imputation takes what? A day or two of compute time? Just re-do it.
Comment: How to access TCGA samples that were treated with a specific drug? by Qroid ▴ 40
Thanks. Really appreciate your help with this. This site is such a great resource. The methods aren't totally clear on how they're gettin…
Comment: How to access TCGA samples that were treated with a specific drug? by GenoMax 142k
It is possible that these drugs may not have been directly used in TCGA. Authors could have looked for mutations known to be acted on by th…
Comment: Does comparing two different groups to a common third group introduce bias in th by Qroid ▴ 40
Are you thinking about a specific scenario? If so, can you provide more info? I think the basic answer is that, yes, your choice of group C…
Answer: Bedtools merge minimum overlap? by harold.smith.tarheel ★ 4.9k
Bedtools [intersect][1] allows you to specify the fraction of overlap between two BED (or BAM) files using the F/f/r flags. You could split…
Comment: How to access TCGA samples that were treated with a specific drug? by Qroid ▴ 40
Sorry, I should have been more specific. By "that list" I mean what's populated in the Therapeutic Agents tab when no filters are applied. …
Comment: Bedtools merge minimum overlap? by bk11 ★ 2.4k
Not sure if you wanting to do like this- cat your_input.bed chr1 100 200 region1 + chr1 180 300 regi…
Comment: Extract gRNA sequence using cutadapt by GenoMax 142k
If you know what the boundaries of your construct look like then trim the left-end of the read using the tag on that end (`ktrim=l`). Then …
Comment: Extract gRNA sequence using cutadapt by gernophil ▴ 80
> Not every read in your data is going to match a guide. That is also true for sure. Let me clarify what I mean. For every read you should…
Answer: VG : No reference-sense paths available in the graph; falling back to generic pa by anovak ▴ 120
If your GFA has paths in it that are P lines with names that don't include a sample name, contig name, and separators, then those are what …
Comment: Extract gRNA sequence using cutadapt by GenoMax 142k
> if you look at a fastq file you should be able to tell for every read definitely, if it has a perfect match for a guide in it and what gu…
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