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116,647 results • Page
2 of 2333
Sort: Rank
Rank
Views
Votes
Replies
0
votes
0
replies
168
views
How to visualize/predict the final transcript from Delly output?
WGS
DELLY
3 days ago by
simplitia
▴ 130
0
votes
1
reply
207
views
Normalize scRNAseq data to housekeeping genes to compare several datasets
RNA-sequencing
housekeeping
Single-cell
normalization
updated 3 days ago by
ATpoint
82k • written 3 days ago by
AaronJaime
• 0
0
votes
2
replies
273
views
BLAST using both nucleotides and taxonomic local databases
blast
ncbi
taxid
taxonomy
updated 3 days ago by
GenoMax
141k • written 3 days ago by
Begonia_pavonina
▴ 150
0
votes
2
replies
303
views
why renaming Idents in Seurat object doesn't work?
Seurat
RenameIdents
R
updated 3 days ago by
Ram
43k • written 3 days ago by
Assa Yeroslaviz
★ 1.8k
1
vote
2
replies
305
views
ScRNAseq-How to correctly choose cell type marker genes
cellAssign
cell-markers
1 day ago by
Francesco
▴ 10
1
vote
6
replies
410
views
ScRNA data question
scRNA
Vlnplot
Samples
1 day ago by
starswillfade
▴ 10
0
votes
0
replies
162
views
Is there a way to increase the automatic label text size in Cytoscape?
Cytoscape
3 days ago by
avocado123
• 0
0
votes
0
replies
157
views
News:
Master Meta-analysis with R (May 13-16) - Online!
Meta-Analysis
Statistics
R
updated 3 days ago by
Ram
43k • written 3 days ago by
carlopecoraro2
★ 2.5k
0
votes
0
replies
149
views
How to calculate correlation coefficient for chipseq?
chipseq
bigwigsummary
correlation
3 days ago by
Emily
▴ 10
1
vote
0
replies
162
views
How to calculate reliable Ka/Ks or dN/dS ratio for genes of interest from VCF file
dnds
kaks
VCF
3 days ago by
rohitsatyam102
▴ 850
0
votes
0
replies
148
views
how to read graph_test output of monocle 3
monocle3
3 days ago by
synat.keam
▴ 100
0
votes
0
replies
177
views
Job:
CTO/founding engineer role at Voyant Bio
engineer
3 days ago by
Assaf
• 0
0
votes
1
reply
477
views
GAPIT p-value significance threshold
GAPIT
p-value
GWAS
updated 3 days ago by
ginellegrenier
• 0 • written 4 months ago by
Clayton
• 0
3
votes
4
replies
324
views
Why gatk VariantAnnotator required bam and coverage files
gatk
VariantAnnotator
3 days ago by
QX
• 0
2
votes
1
reply
222
views
Finding orthologs between genome error
OMA
OMAbrowser
updated 3 days ago by
Adrian Altenhoff
★ 1.1k • written 4 days ago by
hemantcnaik
• 0
0
votes
0
replies
146
views
Why not use iBAQ for calculating differential abundance of proteins?
protein
maxquant
3 days ago by
Aspire
▴ 300
2
votes
5
replies
284
views
Figures are disappeared from html report of SnpEff
HTML
SnpEff
1 hour ago by
analyst
▴ 30
0
votes
3
replies
277
views
Highest variable features in single cell data
single-cell
updated 2 days ago by
bk11
★ 2.4k • written 4 days ago by
Kazo
• 0
0
votes
1
reply
213
views
Network Alignment
plugin
Cytoscape
CytoMCS
updated 4 days ago by
Scooter
▴ 280 • written 4 days ago by
Akash D
▴ 40
2
votes
4
replies
2.4k
views
How could I generate a gi_taxid_nucl.dmp file similar to the one previously hosted by NCBI?
ncbi
metagenomics
centrifuge
gi_taxid_nucl.dmp
taxonomy
updated 4 days ago by
ZhangYuanfeng
• 0 • written 2.4 years ago by
Frazier Baker
• 0
1
vote
3
replies
328
views
PCA plot
DESeq2
PCAplot
updated 13 hours ago by
LauferVA
4.2k • written 4 days ago by
Aaliya
▴ 10
4
votes
4
replies
391
views
Tutorial:
how to combine multiple RNAseq count files into a single dataframe in R and unix
Unix
RNAseq
R
updated 1 day ago by
BioinfGuru
★ 1.7k • written 4 days ago by
Ming Tommy Tang
★ 3.9k
0
votes
4
replies
2.7k
views
Lositan freezing when generating selection table
LOSITAN
updated 4 days ago by
evawillms183
• 0 • written 8.3 years ago by
andrepleao
• 0
0
votes
0
replies
144
views
Can you give me an example of a small globular protein (PDB) that has disordered alpha-carbons?
protein
pdb
alpha-carbon
4 days ago by
4fzcgueyp5
• 0
2
votes
3
replies
318
views
clustalo - align milions of short seq, memory issue
memory
clustalo
updated 6 days ago by
Mensur Dlakic
★ 27k • written 6 days ago by
emmanouil.a
▴ 120
0
votes
2
replies
245
views
Annovar using R package
Annovar
gnomAD
R
3 days ago by
DKA
▴ 40
0
votes
2
replies
223
views
Creating Synthetic Sequences for a ML Model
DNA
ML
updated 4 days ago by
Mensur Dlakic
★ 27k • written 4 days ago by
biochugs
• 0
8
votes
8
replies
588
views
PCA plot (Suggestions Needed)
DESeq2
PCAplot
updated 4 days ago by
swbarnes2
14k • written 5 days ago by
Aaliya
▴ 10
1
vote
8
replies
813
views
Adding CB tag to bam file
samtools
bam
updated 3 days ago by
Pierre Lindenbaum
161k • written 10 days ago by
Maria
• 0
1
vote
10
replies
944
views
Troubleshooting RNA-seq data with DNA contamination
RNA-seq
DESeq2
DNA-contamination
4 days ago by
DKA
▴ 40
0
votes
2
replies
813
views
AGeNT LocatIt
AGeNT
LocatIt
WES
UMI
duplicates
updated 4 days ago by
barslmn
★ 2.1k • written 2.8 years ago by
jhy
▴ 10
0
votes
5
replies
334
views
Is it possible to get a list of representative genomes from a past RefSeq release?
representative
ncbi
asembly
refseq
updated 3 days ago by
GenoMax
141k • written 4 days ago by
Bertalan_Takacs
▴ 90
0
votes
0
replies
137
views
One error problem occurred caused by data type during using GDCprepare to handle BRCA data
TCGA
GDCprepare
updated 4 days ago by
Ram
43k • written 4 days ago by
glaciya2018
• 0
0
votes
0
replies
118
views
News:
8th Berlin Summer School in NGS Data Analysis - Apply Now
DNA-seq
variant-calling
RNA-seq
illumina
transcriptomics
updated 3 days ago by
Ram
43k • written 4 days ago by
David Langenberger
11k
0
votes
0
replies
304
views
roary not working with pgap output gff files with fasta sequence but works with prokka's gff outputs
pangenome
PGAP
NCBI
roary
Prokka
4 days ago by
pramach1
▴ 40
0
votes
13
replies
3.5k
views
6 follow
Tax4Fun2 - error
tax4fun
silva
blast
updated 4 days ago by
drstalinantony28
• 0 • written 2.2 years ago by
ymj
▴ 10
0
votes
1
reply
153
views
GEMMA GWAS how to specify factor or numerical for covariates
covariate
GEMMA
GWAS
updated 4 days ago by
Sofia
• 0 • written 4 days ago by
mawigoj318
• 0
0
votes
0
replies
139
views
Sequence BLAST in plus/minus and plus/plus
blast
CRISPR
strand
sequencing
4 days ago by
salias
• 0
4
votes
7
replies
465
views
To get p-values for the TPM
P-value
TPM
RSEM
updated 3 days ago by
dsull
★ 5.9k • written 5 days ago by
VITALA
• 0
0
votes
5
replies
331
views
different FeatureCounts output for the same data
fpkm
Counts
Rsubread
rna-seq
updated 1 day ago by
Istvan Albert
100k • written 4 days ago by
sehriban.buyukkilic
▴ 10
0
votes
0
replies
119
views
Alternative splicing analysis using SUPPA tool - how to normalise counts?
rna-seq
single-cell
suppa
alternative-splicing
updated 4 days ago by
Ram
43k • written 4 days ago by
newuser2024
• 0
1
vote
2
replies
224
views
alignment result
RNA-seq
samtools
hisat2
4 days ago by
ahmad.sajad4541
• 0
0
votes
0
replies
116
views
News:
hands-on introduction to generalized linear models (GLMs) using R
Generalized-Linear-Models
GLM
R
updated 4 days ago by
Ram
43k • written 5 days ago by
carlopecoraro2
★ 2.5k
0
votes
0
replies
102
views
Is result interpretation of the modelHomotypic function of the DoubletFinder correct?
doublet
DoubletFinder
Seurat
modelHomotypic
updated 4 days ago by
Ram
43k • written 5 days ago by
Jeyong
• 0
0
votes
2
replies
230
views
Wilcox test using data slot (log normalized count values) of scRNA-seq between two groups
scRNAseq
4 days ago by
mropri
▴ 150
2
votes
2
replies
224
views
HaplotypeCaller - only SNPs
HaplotypeCaller
GATK
updated 5 days ago by
analyst
▴ 30 • written 5 days ago by
lorena9132
• 0
0
votes
0
replies
110
views
AttributeError: module 'scvelo' has no attribute 'pp
loomfile
scvelo
scipy
annData
numpy
5 days ago by
Kash
▴ 110
7
votes
2
replies
274
views
Gene set enrichment analysis differences between 2020 and 2024
Gene-ontology
updated 5 days ago by
geneontologyhelp
▴ 390 • written 6 days ago by
catherine.teyssier
• 0
1
vote
1
reply
213
views
Blasting two protein sequences vs two nucleotide sequences
gene-hunting
blast
updated 5 days ago by
Istvan Albert
100k • written 6 days ago by
Abeer
• 0
2
votes
4
replies
312
views
Should I use unpaired reads from trimmomatic
RNA-seq
QC
Trimmomatic
updated 5 days ago by
swbarnes2
14k • written 6 days ago by
dxj294
• 0
116,647 results • Page
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Answer: NGS forensics: how to know if data is fabricated
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Comment: NGS forensics: how to know if data is fabricated
NGS forensics: how to know if data is fabricated
Comment: NGS forensics: how to know if data is fabricated
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Answer: NGS forensics: how to know if data is fabricated
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Comment: NGS forensics: how to know if data is fabricated
by
i.sudbery
19k
I don't follow your argument for distinguishing between genomic contamination and freud. A data set being bad in terms of genomic contamin…
Comment: What does it mean single base resolution in sequencing?
by
jinyu
▴ 10
Thank you for your detail and great explanation. I do need more time to understand these. And there is still something compuzzling me. For …
Answer: Is it possible to bulk download files from GEO repository?
by
atharvakarkare14
▴ 10
Try using this library [GEOparse][1] [1]: https://github.com/guma44/GEOparse
Answer: How many reads for WGS Sequencing?
by
Ruqaiya
• 0
OK. Problem solved. The problem was from my end. Thank you both for helping !!
Comment: NGS forensics: how to know if data is fabricated
by
Mensur Dlakic
★ 27k
Appreciate the suggestion, but that ship has sailed and reached the other shore. This happened 10+ years ago. To the best of my knowledge n…
Comment: Figures are disappeared from html report of SnpEff
by
analyst
▴ 30
Files are not opening in any of the computer (ubuntu/windows). I rereun snpEff annotation but still output html files are having missing im…
Comment: Figures are disappeared from html report of SnpEff
by
analyst
▴ 30
I reinstalled nodejs: > sudo apt-get install nodejs Its already up to date with version v10.19.0
Answer: NGS forensics: how to know if data is fabricated
by
Prash
▴ 280
Mensur, if I were you, I'd probably contact the CA directly and check with him whether they have reproduced the works recently. There coul…
Comment: NGS forensics: how to know if data is fabricated
by
noodle
▴ 560
Working on it - it's actually a bit difficult. Low coverage of ribosomal proteins. Low coverage of housekeeping genes ...I'm trying to find…
Comment: NGS forensics: how to know if data is fabricated
by
noodle
▴ 560
I'm a bit against pubpeer - have you ever posted there? My gripe with them is that the posts are heavily moderated and EDITED to the point…
Comment: NGS forensics: how to know if data is fabricated
by
Philipp Bayer
8.4k
Thanks Mensur! I'd add that in 9 out of 10 cases, you won't receive a reply to a request for raw data. That's just how scientists are, does…
Comment: How many reads for WGS Sequencing?
by
Ruqaiya
• 0
I had downloaded the two files in a similar manner actually.
Comment: How many reads for WGS Sequencing?
by
Ruqaiya
• 0
ok, i'll try to use fastp. I have only used trimmomatic and cutadapt till now and they dont identify on its own. Except the graph in fastqc…
Comment: How many reads for WGS Sequencing?
by
Ruqaiya
• 0
got it! even if i'm doing the assembly for paired end with both the files do i have set the threshold to 1000bp ?
Comment: NGS forensics: how to know if data is fabricated
by
Philipp Bayer
8.4k
i've been in our org trying to push such a detector, our use-case is much more limited: I want to make simulated eDNA libraries as real as …
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